清肺抗敏合剂下调RORγt及SOCS1/3表达改善哮喘小鼠炎症反应机制研究  

Mechanism of Qingfei Kangmin mixture down-regulating RORγt and SOCS1/3 expression to improve inflammatory response in asthmic mice

作　　者：刘智 李巧慧 唐菲 谢辉辉 杨蕾 LIU Zhi;LI Qiaohui;TANG Fei;XIE Huihui;YANG Lei(Hengshui People’s Hospital,Hengshui 053099,China)

机构地区：[1]衡水市人民医院,河北衡水053099

出　　处：《陕西中医》2023年第8期1010-1014,1026,共6页Shaanxi Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81704118);河北省卫生健康委员会医学科学研究项目(20200247)。

摘　　要：目的:研究清肺抗敏合剂下调维甲酸孤儿核受体γt(RORγt)及信号传导抑制因子1/3(SOCS1/3)表达改善呼吸道合胞病毒(RSV)诱发哮喘小鼠炎症反应的影响。方法:将BALB/c小鼠随机分为对照组、模型组、地塞米松组和清肺抗敏合剂低、中、高剂量组。通过RSV诱发、白蛋白致敏建立哮喘小鼠模型。末次激发后24 h,吸入乙酰胆碱(Mch)测定气道的相对阻力;制备肺泡灌洗液(BALF),用酶联免疫吸附法(ELISA)测定BALF中CD31、免疫球蛋白E(IgE)、白介素-4(IL-4)、肿瘤坏死因子-α(TNF-α)水平,采用比色法测定髓过氧化物酶(MPO)含量;计数炎症细胞。采用RT-PCR法检测肺组织中RORγt mRNA水平。采用qPCR法测定肺组织中SOCS1/3 mRNA表达水平。结果:地塞米松组及清肺抗敏合剂不同剂量组小鼠吸入梯度浓度的Mch后气道阻力与模型组比较显著降低(均P<0.05);地塞米松和不同剂量清肺抗敏合剂均可以改善小鼠肺组织的病理学损伤,缓解炎性细胞浸润。地塞米松组及清肺抗敏合剂不同剂量组小鼠病理半定量评分显著低于模型组(均P<0.05),且随着剂量的增加评分降低。与模型组比较,地塞米松组及清肺抗敏合剂不同剂量组小鼠中性粒细胞、淋巴细胞、嗜酸性粒细胞和巨噬细胞数量显著降低(均P<0.05),小鼠BALF中CD31、IgE、IL-4、MPO和TNF-α含量显著降低(均P<0.05),肺组织RORγt及SOCS1/3 mRNA表达水平显著降低(均P<0.05)。结论:清肺抗敏合剂可以通过下调RORγt及SOCS1/3 mRNA表达改善呼吸道合胞病毒诱发哮喘小鼠炎症反应。Objective:To study the effect of Qingfei Kangmin mixture down-regulating the expression of RORγt and SOCS1/3 to improve the inflammatory response of asthmatic mice induced by respiratory syncytial virus.Methods:BALB/c mice were randomly divided into control group,model group,dexamethasone group and low,medium and high dose groups of Qingfei Kangmin mixture.Asthma mouse model was established by respiratory syncytial virus(RSV)induction and albumin sensitization.Twenty-four hours after the last excitation,the relative resistance of airway was measured after inhaling acetylcholine(Mch).Mice were anesthetized and broncho-alveolar lavage fluid(BALF)was prepared.The contents of CD31,IgE,IL-4 and TNF-αin BALF were determined by enzyme-linked immunosorbent assay(ELISA),and myeloperoxidase(MPO)was determined by colorimetry,and inflammatory cells were counting.The level of RORγt mRNA in lung tissue was detected by RT-PCR.The expression level of SOCS1/3 mRNA in lung tissue was determined by qPCR method.Results:Compared with the model group,the airway resistance of dexamethasone group and Qingfei Kangmin mixture group with different doses decreased significantly after inhaling Mch with gradient concentration(all P<0.05).Dexamethasone and different doses of Qingfei Kangmin mixture can improve the pathological injury of mouse lung tissue and relieve inflammatory cell infiltration.The semi-quantitative scores of dexamethasone group and Qingfei Kangmin mixture group were significantly lower than those of model group(all P<0.05),and the score decreased with increasing dose.Compared with model group,the number of neutrophils,lymphocytes,eosinophils and macrophages in dexamethasone group and different doses of Qingfei Kangmin mixture group were significantly decreased(all P<0.05),the contents of CD31,IgE,IL-4,MPO and TNF-αin BALF were significantly decreased(all P<0.05),the expression levels of RORγt and SOCS1/3 mRNA in lung tissue were significantly decreased(all P<0.05).Conclusion:Qingfei Kangmin mixture can improve the infl

关 键 词：哮喘 呼吸道合胞病毒 炎症反应 清肺抗敏合剂 维甲酸孤儿核受体γt 信号传导抑制因子1/3 

分 类 号：R256.12[医药卫生—中医内科学]