基于柱前衍生高效液相色谱法测定蚕蛾中丙氨酸含量的研究  

Determination of alanine in silkworm moth based on precolumn derivatization

作　　者：叶花 杨超 段青青 周瑜聘 姚隆丹 王雅婷 张玺雅 段秀俊 Ye Hua;Yang Chao;Duan Qingqing;Zhou Yupin;Yao Longdan;Wang Yating;Zhang Xiya;Duan Xiujun(Department of Traditional Chinese Medicine,Shanxi Pharmaceutical Vocational College,Taiyuan 030031,China;不详)

机构地区：[1]山西药科职业学院中药系,太原030031 [2]山西中医药大学中药与食品工程系 [3]山西中医药大学第一附属医院药剂科制剂室

出　　处：《山西医药杂志》2023年第11期816-819,共4页Shanxi Medical Journal

基　　金：山西省中药材、中药饮片、地方标准研究项目(2011002A)。

摘　　要：目的建立柱前衍生蚕蛾中丙氨酸含量的测定方法,为不同产地蚕蛾品质评价和质量控制实验依据。方法采用柱前衍生高效液相色谱法。色谱柱为Diamonsil C18色谱柱(4.6 mm×200 mm,5μm);柱温20℃;流动相A为乙腈-水(1∶1),流动相B为0.05 mol/L醋酸钠缓冲液(pH=6.4),梯度;流速1.0 ml/min;检测波长为360 nm。结果丙氨酸进样量在0.04~0.2μg范围内呈良好线性,回归方程为Y=274444X+9597.5;相关系数r=0.9996。结论建立的含量测定方法专属性强、准确度高,重复性良好,可用于蚕蛾药材的质量控制。Objective To establish a method for the determination of alanine in silkworm moth derived from precolumn,so as to provide experimental basis for quality evaluation and quality control of silkworm moth from different habitats.Methods High performance liquid chromatography with precolumn derivatization was used.The chromatographic column is Diamonsil C18 chromatographic column(4.6 mm×200 mm,5μm),column temperature:20℃.Mobile phase A is acetonitrile water(1∶1),mobile phase B is 0.05 mol/L sodium acetate buffer(pH=6.4),gradient;The flow rate is 1.0 ml/min.The detection wavelength is 360 nm.Results The alanine injection volume showed good linearity in the range of 0.04~0.2μg,and the regression equation was Y=274444X+9597.5.Correlation coefficient r=0.9996.Conclusion The method is specific,accurate and reproducible,and can be used for the quality control of silkworm moth.

关 键 词：丙氨酸 色谱法 高压液相 蚕蛾科 

分 类 号：R284.1[医药卫生—中药学]