基于生物信息学的肩袖撕裂与绝经后骨质疏松症的共病研究  

Study on the Comorbidity of Rotator Cuff Tear and Postmenopausal Osteoporosis Based on Bioinformatics

作　　者：汪青[1] 钱胤华 钱平康[1] 徐锋[1] WANG Qing;QIAN Yin-hua;QIAN Ping-kang;XU Feng(Department of Orthopedics,Kunshan Hospital of Chinese Medicine,Kunshan 215300,Jiangsu,China)

机构地区：[1]昆山市中医医院骨科,江苏昆山215300

出　　处：《医学信息》2023年第15期6-11,共6页Journal of Medical Information

基　　金：苏州市临床重点病种诊疗技术专项项目(编号:LCZX202127)。

摘　　要：目的探讨肩袖撕裂(RCT)与绝经后骨质疏松症(PMOP)之间的关系,为肩袖撕裂及绝经后骨质疏松症的共病研究提供新的思路。方法收集自2021年1月1日-2022年6月30日因肩袖撕裂住院的绝经后女性患者的骨密度,并进行分析。应用生物信息学的方法,构建PMOP与RCT共同靶基因的PPI网络,使用cytoscape软件插件cytohubba按照Degree筛选筛选核心基因,并使用R语言进行GO及KEGG富集分析。结果共入组受试者104例,骨量异常共67例,占64.42%,其中骨质疏松症为17例,占16.35%。60岁以上人群,骨质疏松症约占1/3。通过查询数据库共获得绝经后骨质疏松症相关基因共967个,肩袖撕裂相关基因共281个,共同靶点152个。筛选获取前20位核心靶点,包括:信号转导和转录激活因子3(STAT3)、白介素6(IL-6)、血管内皮生长因子A(VEGFA)、金属基质蛋白酶2(MMP2)等。GO生物学过程主要集中在腺体发育、细胞对氧化应激的反应等,KEGG主要富集在癌症中的蛋白聚糖、雌激素信号通道等。结论肩袖撕裂和绝经后骨质疏松症共病在临床常见,两者具有一定的相关性。未来可以从炎症、血管再生、氧化应激和雌激素相关信号通道进行研究。Objective To explore the relationship between rotator cuff tear(RCT)and postmenopausal osteoporosis(PMOP),and to provide new ideas for the study of the comorbidity of rotator cuff tear and postmenopausal osteoporosis.Methods Bone mineral density of postmenopausal women hospitalized with rotator cuff tears from January 1,2021 to June 30,2022 were collected and analyzed.Using the method of bioinformatics,the PPI network of common target genes of PMOP and RCT was constructed.The core genes were screened according to degree by using cytoscape software cytohubba,and the enrichment analysis of GO and KEGG was carried out by using R language.Results A total of 104 subjects were enrolled,of which 67 cases were abnormal bone mass,accounting for 64.42%,including 17 cases of osteoporosis,accounting for 16.35%.Osteoporosis accounts for about 1/3 of people over 60 years old.A total of 967 genes related to postmenopausal osteoporosis,281 genes related to rotator cuff tear and 152 common targets were obtained by querying the database.The top 20 core targets were screened,including signal transducer and activator of transcription 3(STAT3),interleukin 6(IL-6),vascular endothelial growth factor A(VEGFA),matrix metalloproteinase 2(MMP2),etc.GO biological processes mainly focus on gland development,cellular response to oxidative stress,etc.KEGG was mainly enriched in proteoglycans in cancer,estrogen signaling pathway,etc.Conclusion Rotator cuff tear and postmenopausal osteoporosis are common in clinic,and there is a certain correlation between them.In the future,we can study the signal channels related to inflammation,angiogenesis,oxidative stress and estrogen.

关 键 词：绝经后骨质疏松症 肩袖撕裂 共病 信号转导和转录激活因子3 白介素6 血管内皮生长因子A 金属基质蛋白酶2 

分 类 号：R681[医药卫生—骨科学]