光谱共焦显微成像技术与应用  

Techniques and Applications of Chromatic Confocal Microscopy

作　　者：邵谭彬 杨克成[1] 夏珉[1] 郭文平[1] Shao Tanbin;Yang Kecheng;Xia Min;Guo Wenping(School of Optical and Electronic Information,Huazhong University of Science and Technology,Wuhan 430074,Hubei,China)

机构地区：[1]华中科技大学光学与电子信息学院,湖北武汉430074

出　　处：《激光与光电子学进展》2023年第12期1-19,共19页Laser & Optoelectronics Progress

基　　金：国家科技部重点研发专项(2022YFB3206000)。

摘　　要：光谱共焦显微成像(CCM)技术基于色差共焦原理,利用不同波长的焦点位置不同实现深度测量,并使用共聚焦设置滤除离焦光以及杂散光从而提高信噪比。首先,介绍CCM的基本原理以及不同扫描方案。然后,对CCM的发展历程进行梳理,并阐述CCM的国内外研究进展。针对光学设计、信号产生模型、光谱数据处理、减小串扰等关键问题,对相关的研究方案进行了总结。凭借无损检测、高分辨率、高信噪比、层析成像等诸多优势,CCM技术在生物医学、工业检测等领域得到广泛应用。Based on the principle of chromatic aberration confocal microscopy,the chromatic confocal microscopy(CCM)technology utilizes different focal positions of different wavelengths to achieve effective depth measurement;moreover,CCM employs a confocal setting to filter out defocused and stray light to improve the signal-to-noise ratio.This paper first introduces the basic principles of CCM and different scanning schemes,then reviews the development of CCM,and expounds the research progress of CCM at home and abroad.Considering the key issues such as optical design,signal generation model,spectral data processing,and crosstalk reduction,this paper summarizes relevant research schemes.CCM technology has several advantages such as nondestructive testing,high resolution,high signal-to-noise ratio,and effective tomography.Thus,it can be broadly utilized in various fields,including the biomedical,industrial testing,and other fields.

关 键 词：三维成像 光谱共焦显微成像 共聚焦显微成像 超高分辨率 层析成像 

分 类 号：TH742[机械工程—光学工程]