超分辨荧光显微成像的若干研究进展  

Research Progress of Super-Resolution Fluorescence Microscopy

作　　者：卫奥尼 秦成兵[1,2] 董帅 孟新钦 宋蕴睿 李向东 梁喜龙[1,3] 张国峰 陈瑞云[1] 胡建勇[1] 杨志春[1] 霍建忠 肖连团 贾锁堂[1] Wei Aoni;Qin Chengbing;Dong Shuai;Meng Xinqin;Song Yunrui;Li Xiangdong;Liang Xilong;Zhang Guofeng;Chen Ruiyun;Hu Jianyong;Yang Zhichun;Huo Jianzhong;Xiao Liantuan;Jia Suotang(State Key Laboratory of Quantum Optics and Quantum Optics Devices,Institute of Laser Spectroscopy,Collaborative Innovation Center of Extreme Optics,Shanxi University,Taiyuan 030006,Shanxi,China;College of Physics,Taiyuan University of Technology,Taiyuan 030024,Shanxi,China;Taiyuan Central Hospital of Shanxi Medical University,Taiyuan 030009,Shanxi,China)

机构地区：[1]山西大学激光光谱研究所,量子光学与光量子器件国家重点实验室,极端光学协同创新中心,山西太原030006 [2]太原理工大学物理学院,山西太原030024 [3]山西省太原市中心医院,山西太原030009

出　　处：《激光与光电子学进展》2023年第11期148-171,共24页Laser & Optoelectronics Progress

基　　金：国家重点研发计划(2022YFA1404201);国家自然科学基金(U22A2091,62222509,62127817,62075120,62075122)。

摘　　要：超分辨荧光显微成像技术可以突破光学衍射极限,为研究亚细胞结构乃至蛋白质等生物大分子的内部动力学过程提供了极其重要的研究工具,因此自发明以来就被生物及医学领域的科研工作者所关注和应用,特别是近年来生命科学和生物技术的突飞猛进,使超分辨荧光显微成像技术得到了前所未有的发展。本文主要阐述四种较新超分辨荧光显微技术的研究进展,即最低光子数超分辨荧光显微成像,波动超分辨荧光显微成像,基于荧光发色团反聚束效应的超分辨显微成像,以及基于深度学习的超分辨显微成像方法。分别从这些超分辨荧光显微技术的基本原理,实验实现方法及相关要求,成像性能及其与相关技术的对比,以及与其他超分辨技术的结合等角度,对近期的研究进展和应用情况进行简要说明,以期给该领域的研究人员提供一些参考。Super-resolution fluorescence microscopy(SRFM),which can bypass the optical diffraction limit,provide an extremely important research tool for investigations of the subcellular structures and the dynamic processes of biological macromolecules.Therefore,since its invention,SRFM has been paid attention to and applied by scientists in the field of biology and medicine.Benefiting from the rapid development of life science and biotechnology in recent years,SRFM has been developed unprecedentedly.In this paper,we will mainly focus on the research progresses of four new kinds of SRFM,including minimal photon fluxes(MINFLUX),super-resolution optical fluctuation imaging(SOFI),superresolution image based on anti-bunching effect,and deep-learning based super-resolution microscopy,respectively.We will briefly describe the recent research progresses and applications of these SRFM techniques from the basic principles,experimental implementation methods and related requirements,imaging performance,and comparison with other technologies,as well as the combination with other super-resolution techniques,to provide some references for researchers in this field.

关 键 词：显微成像 超分辨 荧光 最低光子数 深度学习 反聚束 

分 类 号：O436[机械工程—光学工程]