一种新型苯酚含量表面等离子共振光纤生物传感器  

Novel Surface Plasmon Resonance Fiber-Optic Biosensor for Phenol Concentration

作　　者：李林洋 彭飞 钟年丙[1] 解泉华 汤斌[1] 常海星 钟登杰[2] Li Linyang;Peng Fei;Zhong Nianbing;Xie Quanhua;Tang Bin;Chang Haixing;Zhong Dengjie(Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection,Chongqing Engineering Research Center of Intelligent Optical Fiber Sensing Technology,Chongqing University of Technology,Chongqing 400054,China;College of Chemistry and Chemical Engineering,Chongqing University of Technology,Chongqing 400054,China)

机构地区：[1]重庆理工大学光纤传感与光电检测重庆市重点实验室,智能光纤感知技术重庆市高校工程研究中心,重庆400054 [2]重庆理工大学化学化工学院,重庆400054

出　　处：《光学学报》2023年第12期224-235,共12页Acta Optica Sinica

基　　金：国家自然科学基金面上项目(52176178,51876018);重庆市高校创新研究群体项目(CXQT21035);重庆市教委科学技术研究重大项目(KJZD-M202201101);重庆市研究生科研创新项目(CYS22645)。

摘　　要：为了提高光纤苯酚含量传感器的灵敏度和选择性,构建了一种新型苯酚含量表面等离子共振(SPR)光纤生物传感器。传感器主要由辣根过氧化物酶(HRP)修饰SPR光纤和苯酚选择透过性膜构成。首先在光纤表面聚合聚多巴胺(PDA),用于吸附纳米金成膜并激发SPR效应,随后在金膜表面再次聚合聚多巴胺用于固定HRP,获得HRP修饰SPR光纤。β-环糊精掺杂PEBA2533苯酚选择性聚合物膜固定在HRP修饰光纤表面。水体中苯酚分子自由通过聚合物膜后,吸附在HRP表面,在过氧化氢(H2O2)协助下被氧化生成难溶聚合物,增大HRP膜的折射率,促进传感器共振波长发生漂移,提高其灵敏度。研究表明,传感器对苯酚含量的测量具有高选择性和高灵敏度;在传感器采样时间为300 s时,灵敏度和检测下限分别达到224.84 pm·mmol^(-1)·L和159 nmol/L。Objective As an important chemical raw material,phenol is widely used in the industrial production of pesticides,dyes,plastics,etc.However,phenol is also a major pollutant in environmental groundwater and surface water.Therefore,the development of online sensors for selective and accurate detection of phenol concentration in water is crucial to protect the water environment and human health.In order to realize online detection of the concentration of phenolic compounds in water,electrochemical,photoelectrochemical,and fiberoptic sensors have been widely studied.Among them,the fiberoptic sensor is one of the most promising sensors for online detection of the concentration of phenolic compounds due to its advantages of longdistance transmission,antielectromagnetic interference,quasidistributed measurement,etc.However,fiberoptic sensors with photocatalyst or oxidase have low selectivity for the catalysis or oxidation of phenol,which makes the sensor not selective for the detection of phenol in water.In addition,there is no report on the development of horseradish peroxidase(HRP)-coated fiberoptic phenol concentration sensor.Therefore,it is necessary to develop an HRPcoated fiberoptic sensor with high sensitivity and selectivity for detecting phenol concentration by using silica optical fibers.Methods To increase the sensitivity and selectivity of the fiberoptic sensor for phenol concentration,a novel surface plasmon resonance(SPR)fiberoptic biosensor composed of horseradish peroxidase(HRP)-coated SPR optical fiber and phenol permeable membrane was created.In order to obtain HRPcoated SPR fibers,first,a layer of polydopamine was polymerized on the surface of the optical fibers,which was used to adsorb gold nanoparticles to form a gold film to stimulate the SPR effect,and then the polydopamine was polymerized on the surface of the gold film to immobilize HRP.Second,the phenol permeable membrane prepared with PEBA2533 andβ-cyclodextrin was sealed on the HRPcoated fiber.Third,an online analytical platform for detecting

关 键 词：传感器 苯酚含量 辣根过氧化物酶 表面等离子共振效应 聚合物膜 光纤传感器 选择性 

分 类 号：TN253[电子电信—物理电子学]