Effect of covalent-binding modes of osteogenic-related peptides with artificial carriers on their biological activities in vivo  

作　　者：Cunyang Wang Suxiang Yu Qi Guo Ke Zhang Yuntao Di Xiaoming Li 

机构地区：[1]Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education,Beijing Advanced Innovation Center for Biomedical Engineering,School of Biological Science and Medical Engineering,Beihang University,Beijing 100083,China [2]Department of Neurosurgery,The Fourth Central Hospital of Baoding City,Baoding 072350,China [3]Department of Biomedical Engineering,City University of Hong Kong,Kowloon,Hong Kong SAR 999077,China

出　　处：《Journal of Materials Science & Technology》2023年第9期163-175,共13页材料科学技术（英文版）

基　　金：support from the National Natural Science Foundation of China(Nos.32171345 and 31771042);the Hebei Provincial Natural Science Foundation of China(No.C2022104003);the Fok Ying Tung Education Foundation(No.141039);the Fund of Key Laboratory of Advanced Materials of Ministry of Education;the International Joint Research Center of Aerospace Biotechnology and Medical Engineering,Ministry of Science and Technology of China,and the 111 Project(No.B13003).

摘　　要：Covalent binding between bioactive substances and materials in different ways can significantly improve the bone inductivity and biological activity of bone repair materials.However,there is a lack of systematic understanding of how these binding modes affect biological activities of the active substances.In this study,four kinds of functionalized Multi-walled carbon nanotubes(MWCNTs)were prepared,ensuring the same grafting rate of different functional groups.Subsequently,two kinds of osteogenic-related peptides,bone morphogenetic protein-2 mimicking peptides and osteogenic growth mimicking peptides,were covalently bound to functionalized MWCNTs,ensuring the same molar mass of peptides bound to different functionalized MWCNTs in this process.Then the same amount of functionalized MWC-NTs/Peptides composites were introduced into the scaffolds,and through the ectopic osteogenesis model in rats and calvarial defect model in rabbits,ectopic osteogenesis and bone repair ability of the composites were analyzed.Furthermore,the effects of different covalent binding modes on peptide-induced osteogenesis and bone repair were studied.The results showed that the negative influencing trend of different covalent binding modes of osteogenic-related peptides with artificial carriers on their biological activities was in the order as follows:amide binding(carboxyl)>silane coupling>dopamine bind-ing>amide binding(amino),whose mechanism might be mainly that the covalent binding of peptides with different functional groups resulted in different charges.We believe that the results of this study have important guiding significance for the research and development of bone repair materials covalently bound with bioactive substances.

关 键 词：Osteogenic-related peptides Covalent binding Ectopic osteogenesis Calvarial defect 

分 类 号：TB306[一般工业技术—材料科学与工程] R318.08[医药卫生—生物医学工程]