A HAase/NIR responsive surface on titanium implants for treating bacterial infection and improving osseointegration  

作　　者：Dan Li Danyang Wang Ye He Bailong Tao Xiaoxia Liu Yulu Yang Lu Tan Yuchen Zhang Jingwei Hu Weihu Yang Yu Tang Kaiyong Cai 

机构地区：[1]Key Laboratory of Biorheological Science and Technology,Ministry of Education,College of Bioengineering,Chongqing University,Chongqing 400044,PR.China [2]Laboratory Research Center,The First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,PR.China [3]School of Life Science,Chongqing University,Chongqing 400044,PR.China [4]Department of Orthopedics,The Second Affiliated Xinqiao Hospital of Army Medical University,Chongqing 400030,PR.China

出　　处：《Journal of Materials Science & Technology》2023年第12期93-106,共14页材料科学技术（英文版）

基　　金：financially supported by the National Natu-ral Science Foundation of China (Nos.32071334,51825302 and 21734002);the Natural Science Foundation of Chongqing (Nos.cstc2021jcyj-cxttX0002 and cstc2019jscx-msxmX0160).

摘　　要：Bacterial infection and insufficient osseointegration are critical factors affecting the long-term success of titanium-based implants.Unfortunately,the direct application of antibiotic on Ti implants easily leads to poor cytocompatibility,as well as the production of drug-resistant bacteria.So,in this work,we designed a prospective antibacterial strategy by combining photothermal and ciprofloxacin(CIP).The synergistic effect of photothermal and antibiotic may provide an effective bacteriostatic efficacy without sacrificing osteogenesis at a mild condition of moderate temperature and less antibiotic.Herein,CIP was loaded into mesoporous polydopamine(MPDA)nanoparticles(MPDA@CIP),which were anchored on the surface of titanium and finally covered with sodium hyaluronate-catechol(HAc)coating.The hydrophilic HAc layer could inhibit the early adhesion of bacteria,and some bacteria could secrete bacterial hyaluronidase to accelerate the degradation of HAc.This enabled smart enzyme-triggered release of antimicrobials at the site of infection on-demand and avoided unwanted side effects on normal tissues.In addition,NIR light irradiation had a positive influence on both CIP release and MPDA nanoparticle’s photothermal effect.Moreover,before anchoring MPDA@CIP,by the construction of hydroxyapatite microstructure on Ti sur-face with micro-arc oxidation and alkali heat treatment,the ability of bone formation of Ti could be promoted also.Both in vitro as well as in vivo assays demonstrated that functional Ti has an excellent antibacterial effect and osteogenic ability.

关 键 词：Titanium implant ANTIBIOSIS Hyaluronidase response Mesoporous polydopamine nanoparticles 

分 类 号：TG174.4[金属学及工艺—金属表面处理] R318.08[金属学及工艺—金属学]