单细胞/单细胞核RNA测序分析心脏细胞转录谱推进心脏细胞类型和心脏发育研究  

Single Cell/Nucleus RNA Sequencing Profiles the Cardiac Atlas and Improves Research in Heart Cells and Heart Development

作　　者：文莉雅 雅各布·马西卡 王子赫 王松 梁华敏[1,2] Wen Liya;Jacob Masika;Wang Zihe(Department of Physiology,Hubei Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation,School of Basic Medicine,Huazhong University of Science and Technology,Wuhan 430030,China;National Demonstration Center for Ex perimental Basic Medical Education,Huaxhong University of Science and Technology,Wuhan 430030,China;Department of Medical Ph ysiology,School of Medicine,Kenyatta University,Box 43844-00100 GPO Nairobi,Kenya)

机构地区：[1]华中科技大学基础医学院生理学系,湖北省药物靶点研究与药效学评价重点实验室,武汉430030 [2]华中科技大学国家基础医学实验示范中心,武汉430030 [3]肯雅塔大学医学院生理学系,肯尼亚内罗毕43844-00100

出　　处：《华中科技大学学报（医学版）》2023年第4期554-557,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：科技部国家重点研发计划项目(No.2021YFA1101901);中国大学生创新创业训练项目基金(No.HUST:20210124)。

摘　　要：以单细胞RNA测序(single cell RNA sequencing,scRNA-seq)和单细胞核RNA测序(single-nucleus RNA sequencing,snRNA-seq)进行单细胞/单细胞核转录组学分析,为组织在稳态维持或动态变化过程(如疾病的发展、分化和发育)中提供了一个全面的组织细胞统计可视图。与传统批量分析相比,单细胞/单细胞核为中心的转录组分析更准确地揭示组织内细胞的异质性和细胞内基因调控网络。该文总结了应用sc/snRNA-seq技术分析心脏细胞转录谱的新发现,以及心血管发育的重要新分子机制。Single-cell transcriptomics analysis with single cell and single nucleus RNA sequencing(sc/snRNA-seq)provides a global and unbiased view of tissues cellular demographics both at steady-state and in dynamic processes such as development,differentiation,and progression of diseases.Single cel/nucleus transcriptome analysis reveals the cell heterogeneity more accu-rately than the traditional batch analysis,as well as the regulation network in cells.In this review,we summarize the recent find-ings in the cardiac cellular atlas from various sc/snRNA-seq platforms and describe the latest progress in molecular mechanisms of cardiovascular development and cardiac regeneration.

关 键 词：单细胞RNA测序 单细胞核RNA测序 心肌细胞 心脏发育 

分 类 号：R3[医药卫生—基础医学]