高效液相色谱法测定鸡血浆中L-肉碱含量  

Determination of L-Carnitine in Chicken Plasma by High Performance Liquid Chromatography

作　　者：孙雯可 王佳璐 刘鼎阔 李源 于晓雪 李留安 SUN Wenke;WANG Jialu;LIU Dingkuo;LI Yuan;YU Xiaoxue;LI Liu'an(College of Animal Science and Veterinary Medicine,Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,Tianjin Agricultural University,Tianjin 300392,China;S&E Burgeoning Biotechnology(Tianjin)Co.,Ltd.,Tianjin Key Laboratory of Biological Feed Additive Enterprise,Tianjin 300383,China)

机构地区：[1]天津农学院动物科学与动物医学学院,天津市农业动物繁育与健康养殖重点实验室,天津300392 [2]鼎正新兴生物技术(天津)有限公司,天津市生物饲料添加剂企业重点实验室,天津300383

出　　处：《食品研究与开发》2023年第15期149-154,共6页Food Research and Development

基　　金：天津市科技计划项目(21YDTPJC00580、22YDTPJC00990、18ZXBFNC00310);天津市教委科研项目(2021KJ108);天津市“131”创新型人才团队建设项目(20180318)。

摘　　要：试验旨在建立高效液相色谱(high-performance liquid chromatography,HPLC)法检测鸡血浆中L-肉碱含量的方法。样品采用乙腈甲醇混合液(9∶1,体积比)提取,涡旋振荡,离心,定容后,经0.22μm滤膜过滤,使用ACE Excel 5Super C_(18)色谱柱进行分离。流动相为乙腈-5 mmol/L庚烷磺酸钠(加入0.1%磷酸)水溶液(15∶85,体积比),流速0.6 mL/min,柱温30℃,检测波长210 nm,进样量20μL。结果显示,该条件下,L-肉碱质量浓度与其峰面积在0.01~0.60 mg/mL内,具有良好的线性关系,相关系数为0.9998,检测限为0.005 mg/mL,定量限为0.017 mg/mL,平均回收率为97.393%~98.703%,相对标准偏差(relative standard deviation,RSD)为0.8%(n=6)。使用该方法测得鸡血浆中L-肉碱峰型良好,无杂峰干扰。研究表明该方法成本低,操作简单,有较高的回收率,适用于鸡血浆中L-肉碱的含量测定。The aim of this study was to establish a method for the determination of L-carnitine in chicken plasma by high-performance liquid chromatography(HPLC).The samples were extracted with a mixture of acetonitrile and methanol(9∶1,volume ratio),vortexed,centrifuged,and diluted to a constant volume.After filtration with a 0.22μm filter membrane,the samples were separated using an ACE Excel 5 Super C_(18) chromatographic column.The detection wavelength was 210 nm.The mobile phase was acetonitrile-5 mmol/L sodium heptanesulfonate(adding 0.1%phosphoric acid)aqueous solution(15∶85,volume ratio),the flow rate was 0.6 mL/min,the column temperature was 30℃,the detection wavelength was 210 nm,and the injection volume was 20μL.The results showed that under this condition,the mass concentration of L-carnitine and its peak area had a good linear relationship in the range of 0.01-0.60 mg/mL,the correlation coefficient was 0.9998,the detection limit was 0.005 mg/mL,and the limit of quantitation was 0.017 mg/mL.The average recovery rate was 97.393%-98.703%,and the relative standard deviation(RSD)was 0.8%(n=6).Using this method,the L-carnitine peak in plasma was good and there was no impurity peak interference.The results showed that the method had low cost,simple operation and high recovery rate,which was suitable for the determination of L-carnitine in chicken plasma.

关 键 词：血浆 L-肉碱 高效液相色谱法 检测 含量 

分 类 号：O657.72[理学—分析化学] TS251.93[理学—化学]