翘嘴鳜miR-125b的时空表达特征及饥饿对其节律性表达的调控  

Analysis of the Spatial-temporal Expression Characteristics of miR-125b in Siniperca chuatsi and the Regulation of Its Rhythmic Expression by Starvation

作　　者：朱鑫[1] 秦家鑫 何雅芝 孟杨杨 丁颖 褚武英[1] 向静[1] 成嘉 陈圆华[1] 张建社[1] ZHU Xin;QIN Jiaxin;HE Yazhi;MENG Yangyang;DING Ying;CHU Wuying;XIANG Jing;CHENG Jia;CHEN Yuanhua;ZHANG Jianshe(Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals,College of Biological and Chemical Engineering,Changsha University,Changsha,410022)

机构地区：[1]长沙学院生物与化学工程学院,水生动物营养与品质调控湖南省重点实验室,长沙410022

出　　处：《基因组学与应用生物学》2023年第1期34-43,共10页Genomics and Applied Biology

基　　金：国家自然科学基金项目(31820103016,32002370);湖南省自然科学基金项目(2021JJ40629);湖南省教育厅创新平台开发基金项目(20K014)共同资助。

摘　　要：为探究翘嘴鳜(Siniperca chuatsi)miR-125b的时空表达特征以及饥饿条件对其节律性表达调控的影响,本研究利用实时定量PCR技术分析了miR-125b在翘嘴鳜不同组织和胚胎不同发育阶段以及饥饿5 d后的昼夜节律表达特征。结果显示,miR-125b在翘嘴鳜的红肌表达最高,在心肌表达次之,在其他组织表达较低;miR-125b在2细胞至32细胞期表达处于最高水平,进入囊胚期后表达降低,并一直保持低表达直至心博期,然后在出膜期表达开始上调。正常投喂时,miR-125b在白肌中的表达无明显的昼夜节律性;而饥饿5 d后白肌中miR-125b的表达呈现明显的昼夜节律,为昼高夜低的节律性振荡;正常投喂和饥饿状态下,Arntl2的表达与miR-125b趋势相反,且用miR-125b抑制剂处理后Arntl2的表达显著上升。研究结果表明miR-125b呈母源性高表达,推测其调控的靶基因可能抑制胚胎的早期发育并且具有广泛的生物学功能;miR-125b可能通过负调控昼夜节律基因Arntl2的表达,参与在饥饿条件下翘嘴鳜肌肉的适应性生理代谢功能的调节。In order to explore the spatial-temporal expression characteristics of miR-125b and the effect of starvation on its rhythm in Siniperca chuatsi, real time quantitative PCR was used to analyze the expression of miR-125b in the embryos of different developmental stages, different tissues and the rhythmic expression after 5 days of starvation. The results showed that the expression of miR-125b was highest in red muscle followed by myocardium, whereas lower in other tissues. The expression of miR-125b in the embryonic development stage showed decreased in the early embryos and then increased in hatching larvae. The expression was at the highest level during the 2 cell stage to 32 cell stage, significantly deceased after entering the blastula stage, and remained low level until heart beating stage, then began to be up-regulated at hatching larvae stage. In normal feeding, the expression of miR-125b in white muscle showed no obviously circadian rhythm. After 5 days of starvation, the expression of miR-125b showed obvious rhythmic oscillation, which rhythmic oscillation was higher in the day and lower in the night, in white muscle. In normal feeding and starvation conditions, the expression of Arntl2 was opposite to miR-125b, and the expression of Arntl2 was significantly increased after treatment with miR-125b antagomir. The results indicated that the expression of miR-125b was maternal in the embryo, suggesting that the target genes regulated by miR-125b may inhibit early embryonic development and have a wide range of biological functions. Furthermore, the results indicated that miR-125b could participate in regulating adaptive physiological metabolism function of S. chuatsi muscle by negatively regulating the expression of circadian gene Arntl2 under starvation stress.

关 键 词：翘嘴鳜 miR-125b 时空表达 昼夜节律 饥饿 

分 类 号：S917.4[农业科学—水产科学]