分子标记辅助选择改良加工番茄的耐旱性  

Drought Tolerance Improvement of Processing Tomato by Molecular Marker Assisted Selection

作　　者：沈心彦 侯晓雷 孙培楠 刘敏敏 唐亚萍 李宁[2] 卢永恩[1] 叶志彪[1] 欧阳波[1] SHEN Xinyan;HOU Xiaolei;SUN Peinan;LIU Minmin;TANG Yaping;LI Ning;LU Yong’en;YE Zhibiao;OUYANG Bo(National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops,College of Horticulture&Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China;Institute of Horticultural Crops,Xinjiang Academy of Agricultural Sciences,Urumchi 830091,China)

机构地区：[1]华中农业大学园艺林学学院,果蔬园艺作物种质创新与利用全国重点实验室,武汉430070 [2]新疆农业科学院园艺作物研究所,乌鲁木齐830091

出　　处：《园艺学报》2023年第7期1429-1443,共15页Acta Horticulturae Sinica

基　　金：国家重点研发计划项目(2022YFE0100900,2018YFD1000800);国家自然科学基金项目(31972416,U1906205,U1503186);国家现代农业产业技术体系建设专项资金项目(CARS-23-A13)。

摘　　要：以耐旱番茄M1040为供体,加工番茄里格尔87-5和M3为受体,利用耐旱分子标记ID68对回交材料进行连续4代选择,最终获得改良系里格尔87-5D和M3D。对改良系的苗期耐旱性进行全面评估,结果表明:改良系在重度干旱后的复水存活率显著高于轮回亲本,旱害指数显著低于轮回亲本;在中度干旱胁迫下,改良系的生长、生理和生化指标全面优于轮回亲本。农艺性状比较结果表明,改良系基本恢复到轮回亲本的遗传背景。Molecular marker-assisted selection was used to improve the drought tolerance of two processing tomato lines(Rigel 87-5 and M3).In this breeding process,a drought-tolerant tomato line(M1040)was used as the donor,while Rigel 87-5 and M3 were used as the recurrent parents.The drought-tolerant molecular marker ID68 was used to select the backcrossing materials for four backcross generations.The drought tolerance of the improved lines(Rigel 87-5D and M3D)at seedling stage was evaluated in detail.The results showed that the survival rate of 87-5D and M3D was significantly higher than that of the recurrent parent after severe drought and rehydration,which can also be reflected by the curve of injury index.The growth,physiological and biochemical indicators under moderate drought stress showed that the improved lines were clearly better than the recurrent parents.Comparison of agronomic traits suggested that the genetic background of the improved lines was restored.

关 键 词：番茄 分子育种 回交 耐旱 供体 

分 类 号：S641.2[农业科学—蔬菜学]