Zinc enhances the cell adhesion,migration,and self-renewal potential of human umbilical cord derived mesenchymal stem cells  

作　　者：Iqra Sahibdad Shumaila Khalid G Rasul Chaudhry Asmat Salim Sumreen Begum Irfan Khan 

机构地区：[1]Dr.Panjwani Center for Molecular Medicine and Drug Research,International Center for Chemical and Biological Sciences,University of Karachi,Karachi 75270,Sindh,Pakistan [2]Department of Biological Sciences,Oakland University,Rochester,MI 48309,United States [3]Stem Cell Research Laboratory(SCRL),Sindh Institute of Urology and Transplantation(SIUT),Karachi 74200,Sindh,Pakistan

出　　处：《World Journal of Stem Cells》2023年第7期751-767,共17页世界干细胞杂志（英文版）（电子版）

基　　金：Supported by Higher Education Commission,Pakistan indigenous fellowship,No.520-149004-2MD6-75(to Ms.Iqra Sahibdad)and No.520-165117-2BS6-21(to Ms.Shumaila Khalid).

摘　　要：BACKGROUND Zinc(Zn)is the second most abundant trace element after Fe,present in the human body.It is frequently reported in association with cell growth and proliferation,and its deficiency is considered to be a major disease contributing factor.AIM To determine the effect of Zn on in vitro growth and proliferation of human umbilical cord(hUC)-derived mesenchymal stem cells(MSCs).METHODS hUC-MSCs were isolated from human umbilical cord tissue and characterized based on immunocytochemistry,immunophenotyping,and tri-lineage differentiation.The impact of Zn on cytotoxicity and proliferation was determined by MTT and Alamar blue assay.To determine the effect of Zn on population doubling time(PDT),hUC-MSCs were cultured in media with and without Zn for several passages.An in vitro scratch assay was performed to analyze the effect of Zn on the wound healing and migration capability of hUC-MSCs.A cell adhesion assay was used to test the surface adhesiveness of hUC-MSCs.Transcriptional analysis of genes involved in the cell cycle,proliferation,migration,and selfrenewal of hUC-MSCs was performed by quantitative real-time polymerase chain reaction.The protein expression of Lin28,a pluripotency marker,was analyzed by immunocytochemistry.RESULTS Zn at lower concentrations enhanced the rate of proliferation but at higher concentrations(>100μM),showed concentration dependent cytotoxicity in hUC-MSCs.hUC-MSCs treated with Zn exhibited a significantly greater healing and migration rate compared to untreated cells.Zn also increased the cell adhesion rate,and colony forming efficiency(CFE).In addition,Zn upregulated the expression of genes involved in the cell cycle(CDC20,CDK1,CCNA2,CDCA2),proliferation(transforming growth factorβ1,GDF5,hypoxia-inducible factor 1α),migration(CXCR4,VCAM1,VEGF-A),and self-renewal(OCT4,SOX2,NANOG)of hUC-MSCs.Expression of Lin28 protein was significantly increased in cells treated with Zn.CONCLUSION Our findings suggest that zinc enhances the proliferation rate of hUC-MSCs decreasing the PDT,and m

关 键 词：Human umbilical cord Mesenchymal stem cells ZINC Cell proliferation In vitro expansion 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]