溴氰虫酰胺对小菜蛾幼虫肠道真菌群落的影响  

Effects of Cyantraniliprole on Gut Fungal Community in Plutella xylostella(L.)Larvae

作　　者：陈华璋[1] 郑苹 田旭 汪汉成 向立刚 李文红[4] Chen Huazhang;Zheng Ping;Tian Xu;Wang Hancheng;Xiang Ligang;Li Wenhong(Institute of Upland Crops,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;Guizhou Academy of Tobacco Science,Guiyang 550081,China;College of Agriculture,Yangtze University,Jingzhou 434000,China;Institute of Plant Protection,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China)

机构地区：[1]贵州省农业科学院旱粮研究所,贵州贵阳550006 [2]贵州省烟草科学研究院,贵州贵阳550081 [3]长江大学农学院,湖北荆州434000 [4]贵州省农业科学院植物保护研究所,贵州贵阳550006

出　　处：《山东农业科学》2023年第7期73-79,共7页Shandong Agricultural Sciences

基　　金：国家自然科学基金项目“肠道微生物对小菜蛾耐受溴氰虫酰胺胁迫的作用与机制”(31860509)。

摘　　要：昆虫肠道共生真菌对宿主的生长发育、食物吸收与消化以及防御等多种生命活动有重要意义。为了解第二代鱼尼丁受体抑制剂溴氰虫酰胺作用小菜蛾后对其肠道真菌群落的影响,本研究采用传统分离培养法和高通量测序技术研究了1 mg/L溴氰虫酰胺浸叶饲喂与未经处理的小菜蛾幼虫肠道真菌群落组成。结果表明,小菜蛾肠道可培养优势真菌主要为青霉菌(Penicillium sp.)和盾壳霉菌(Coniothyrium sp.),经溴氰虫酰胺处理后的小菜蛾肠道可培养真菌还包括曲霉菌(Aspergillus sp.)和黄瓜织球壳菌(Plectosphaerella cucumerina)。高通量测序表明,小菜蛾幼虫肠道优势真菌门为子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、毛霉门(Mucoromycota)等,优势菌属为平脐蠕孢属(Bipolaris)、镰刀菌属(Fusarium)、散尾鬼笔属(Lysurus)、枝孢属(Cladosporium)和赤霉菌属(Gibberella)等。1 mg/L溴氰虫酰胺浸叶饲喂后,小菜蛾肠道中担子菌门、镰刀菌属和散尾鬼笔属等相对丰度显著降低,而平脐蠕孢属相对丰度显著增加;小菜蛾肠道真菌群落丰富度和多样性也有增加但不显著。功能预测表明小菜蛾幼虫肠道真菌群落主要功能为植物病原菌、土壤木材腐生菌以及植物内生菌等类群。可见,溴氰虫酰胺处理后小菜蛾幼虫肠道中真菌种类及其功能无显著变化,仅对其相对丰度和数量有较大影响。Insect intestinal symbiotic fungi are important for host life activities such as growth and devel-opment,food absorption and digestion,and defense.In order to understand the effects of the second-genera-tion ryanodine receptor inhibitor cyantraniliprole on the gut fungal community of Plutella xylostella,traditional culture-dependent method and high-throughput sequencing technique were used to study the gut fungal commu-nity structure of P.xylostella fed with 1 mg/L cyantraniliprole and no.The results showed that the dominant culturable gut fungi of P.xylostella were mainly Penicillium sp.and Coniothyrium sp.,and there were also As-pergillus sp.and Plectosphaerella cucumerina after treated with cyantraniliprole.High-throughput sequencing results showed that the dominant gut fungal phylum were Ascomycota,Basidiomycota and Mucoromycota,and the dominant genera were Bipolaris,Fusarium,Lysurus,Cladosporium and Gibberella,etc.After feeding with 1 mg/L cyantraniliprole,the relative abundance of Basidiomycota,Fusarium and Lysurus significantly de-creased,while that of Bipolaris significantly increased;the richness and diversity of fungal community also in-creased but not significant.Functional prediction indicated that the main functions of fungal community were dominated by plant pathogens,soil and wood saprotropha,and endophytes.In conclusion,there were no sig-nificant changes in the gut fungal species and functions of P.xylostel after fed with 1 mg/L cyantraniliprole,but there were great effects on the fungal relative abundance and amount.

关 键 词：小菜蛾 溴氰虫酰胺 肠道真菌菌群 高通量测序 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]