ZIF-8负载的吲哚菁绿光热治疗常见菌感染的研究  

Study on the Photothermal Treatment of Common Bacterial Infections Using Indocyanine Green-loaded ZIF-8

作　　者：鲁旭 杜亭亭[1] 方远 王晓敏 LU Xu;DU Tingting;FANG Yuan;WANG Xiaomin(Ministry of Health and Medical,General Hospital of Tianjin Medical University,Tianjin 300052,China;State Key Laboratory of Food Nutrition and Safety,College of Food Science and Engineering,Tianjin University of Science and Technology,Tianjin 300457,China;School of Integrative Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津医科大学总医院保健医疗部,天津300052 [2]天津科技大学食品科学与工程学院,食品营养与安全国家重点实验室,天津300457 [3]天津中医药大学中西医结合学院,天津301617

出　　处：《中国药物滥用防治杂志》2023年第7期1204-1209,共6页Chinese Journal of Drug Abuse Prevention and Treatment

摘　　要：目的:探讨ZIF-8@ICG的光热稳定性及对S. aureus和E.coli的光热杀菌效果。方法:制备ZIF-8@ICG纳米复合颗粒并利用紫外光谱对其进行表征;测定不同浓度ZIF-8@ICG溶液(0.05、0.1、0.2、0.5和1 mg/ml)的光热升温曲线以评估其光热转化性能;测定ZIF-8@ICG和ICG的光热循环变化图以对比二者光热循环稳定性;将PBS、ZIF-8、ICG、ZIF-8@ICG溶液分别与1×10^(6) CFU/ml S.aureus和E.coli悬浮菌液混合,每个菌种不同溶液抗菌实验各分为对照组和实验组,实验组均接受808 nm、1.5 W/cm^(2)的近红外光照处理20 min,对照组无光照处理。取样后培养12 h,记录S.aureus和E.coli的菌落数量,并比较各组灭菌效果。结果:紫外吸收光谱证实ICG与ZIF-8形成稳定的ZIF-8@ICG纳米负载结构;ZIF-8@ICG溶液(0.2mg/ml)经过5 min的近红外光照射(808nm,1.5 W/cm^(2))温度可升高30.4℃,浓度升高至1 mg/ml的溶液最高温度可达到72.3℃,而纯水仅升高了2.4℃;经第2个照射周期后,ICG最高温度仅为37.2℃且持续减弱,ZIF-8@ICG纳米复合颗粒在经历了第2、3、4个照射周期后,最高温度分别升高至50℃、47.1℃、43.6℃;经近红外光照射后,ZIF-8@ICG纳米复合颗粒对S.aureus和E.coli的杀菌率分别为99.97%和99.91%,高于ICG对照组,差异有统计学意义(P<0.001)。结论:ZIF-8@ICG纳米复合颗粒具有高效的光热转化性能,经近红外激光照射后对S.aureus和E.coli具有较强的杀菌效果。Objective:To investigate the photothermal stability of ZIF-8@ICG and its photothermal killing effect on S.aureus and E.coli.Methods:ZIF-8@ICG nanoparticles were prepared and characterized using UV spectroscopy.Photothermal heating curves were measured for different concentrations of ZIF-8@ICG solution(0.05,0.1,0.2,0.5,and 1 mg/ml)to evaluate its photothermal conversion performance.The photothermal cycling stability of ZIF-8@ICG and ICG were compared.Suspensions of 1×10^(6) CFU/ml S.aureus and E.coli were mixed with PBS,ZIF-8,ICG,and ZIF-8@ICG solution,respectively.Each bacterial solution was divided into control and experimental groups.The experimental groups were treated with near-infrared light(808 nm,1.5 W/cm^(2))for 20 min,while the control groups received no light treatment.After incubation for 12 h,the number of S.aureusandE.coli colonies was recorded,and the sterilization effect of each group was compared.Results:UV absorption spectroscopy confirmed the formation of a stable ZIF-8@ICG nano-load structure.The ZIF-8@ICG solution(0.2 mg/ml)could be heated to 30.4℃after 5 min of near-infrared light irradiation(808 nm,1.5 W/cm^(2)).The highest temperature of the solution with a concentration of 1 mg/mL could reach 72.3℃,while pure water only increased by 2.4℃.After the second irradiation cycle,the highest temperature of ICG was only 37.2℃and continued to decrease,while the highest temperature of ZIF-8@ICG nano-composite particles increased to 50℃,47.1℃,and 43.6℃after the second,third,and fourth irradiation cycles,respectively.After near-infrared light irradiation,the sterilization rates of ZIF-8@ICG nano-composite particles against S.aureusand E.coli were 99.97% and 99.91%,respectively,which were higher than those of the ICG control group and had statistical significance(P<0.001).Conclusion:ZIF-8@ICG nano-composite particles have efficient photothermal conversion performance and strong sterilization effect against S.aureusandE.coli after near-infrared laser irradiation.

关 键 词：光热疗法 ZIF-8 吲哚菁绿 金黄色葡萄球菌 大肠杆菌 杀菌 

分 类 号：R454[医药卫生—治疗学]