氨海水和5-羟色胺对菲律宾蛤仔解剖配子体外促熟的影响  

Effect of ammonia seawater and 5-HT on the maturation of gametes stripped from Ruditapes philippinarum

作　　者：巫旗生[1] 祁剑飞[1] 罗娟[1] 宁岳[1] 郭香[1] 罗辉玉 曾志南[1] WU Qisheng;QI Jianfei;LUO Juan;NING Yue;GUO Xiang;LUO Huiyu;ZENG Zhinan(Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province,Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources,Fisheries Research Institute of Fujian,Xiamen 361013,China)

机构地区：[1]福建省水产研究所,福建省海洋生物增养殖与高值化利用重点实验室,福建省海洋生物资源开发利用协同创新中心,福建厦门361013

出　　处：《渔业研究》2023年第4期341-345,共5页Journal of Fisheries Research

基　　金：现代农业产业技术体系建设专项(CARS-49);国家海洋水产种质资源库;福建省海洋与渔业结构调整专项(2021HYJG19、2021HYJG01)。

摘　　要：采用解剖配子体外浸泡的方法,研究了氨海水和5-羟色胺(5-HT)对菲律宾蛤仔(Ruditapes philippinarum)配子的促熟作用。结果表明,采用一定浓度的氨海水和5-HT浸泡能促进菲律宾蛤仔卵子的生发泡破裂(Germinal vesicle breakdown,GVBD)(P<0.05),氨海水处理组的受精率优于5-HT处理组。其中,10μmol/L 5-HT浸泡卵母细胞60 min受精率最高,达到28.38%;0.015%氨海水浸泡卵母细胞60 min受精率最高,达到41.28%。To construct the artificial breeding method of R.philippinarum,effects of ammonia seawater and 5-hydroxytryptamine(5-HT)on the maturation of gametes were studied by soaking the dissected gametes in vitro.Results showed that immersion with ammonia seawater and 5-HT significantly stimulated the germinal vesicles breakdown in R.philippinarum(P<0.05).However,the fertilization rate of ammonia seawater treatment group was better than that of 5-HT treatment group.The fertilization rate of oocytes treated with 10μmol/L 5-HT for 60 min was 28.38%.The fertilization rate of oocytes treated with 0.015%ammonia seawater for 60 min was the highest(about 41.28%).

关 键 词：菲律宾蛤仔 卵母细胞 体外促熟 氨海水 5-羟色胺 

分 类 号：S968.31[农业科学—水产养殖]