分子印迹SPR传感器用于骆驼奶粉中β-乳球蛋白的检测  

Molecularly Imprinted SPR Sensor for Detection ofβ-Lactoglobulin in Camel Milk Powder

作　　者：申永帅 胡子凡 朱良宇 王雨晨 何康 王延梅[1] SHEN Yongshuai;HU Zifan;ZHU Liangyu;WANG Yuchen;HE Kang;WANG Yanmei(Department of Polymer Science and Engineering,University of Science and Technology of China,Hefei 230026,China)

机构地区：[1]中国科学技术大学高分子科学与工程系,合肥230026

出　　处：《功能高分子学报》2023年第4期349-357,I0002,共10页Journal of Functional Polymers

基　　金：国家自然科学基金(21674102)。

摘　　要：结合模板分子前定位、表面印迹法和后修饰策略,在金(Au)基底上构建了分子印迹表面等离子体共振(SPR)传感器,并将其用于骆驼奶粉中β-乳球蛋白(BLG)的检测。首先在聚多巴胺(PDA)修饰的金片(PDA/Au)上,通过巯基封端的聚丙烯酸(PAA-SH)固定模板分子BLG,以多巴胺为功能单体和交联剂进行印迹;然后在非印迹部分接枝具有抗蛋白质吸附功能的部分水解聚(2-甲基-2-噁唑啉)(PMOXA-EI),洗脱模板后,得到BLG分子印迹SPR传感器。采用水接触角(WCA)研究了传感器表面亲/疏水的变化,用傅里叶变换红外光谱仪(FT-IR)、原子力显微镜(AFM)、可变角光谱椭偏仪(VASE)对其进行表征。结果表明,该传感器对0.21~10μg/mL的BLG具有良好的线性关系,检测限为0.12μg/mL,能快速灵敏地检测出骆驼奶粉中BLG的质量浓度,回收率为100.2%~100.7%。Combined with template molecular pre-positioning,surface imprinting method,and post-modification strategy,a molecularly imprinted surface plasmon resonance(SPR)sensor was constructed on a gold substrate and used for detection ofβ-lactoglobulin(BLG)in camel milk powder.Firstly,the template molecule BLG was fixed by mercapto-terminated polyacrylic acid(PAA-SH)on the gold substrate which was modified by polydopamine in advance.Then imprinting was performed using dopamine as functional monomer and crosslinking agent.Finally,partially hydrolyzed poly(2-methyl-2-oxazoline)(PMOXA-EI)with anti-protein adsorption function was grafted on the non-imprinted part,and the BLG molecularly imprinted SPR sensor was prepared after eluting the template.The changes of sensor’s surface hydrophilicity/hydrophobicity were studied by water contact angle(WCA)and the characterization of created sensor was performed by Fourier transform infrared spectrometer(FT-IR),atomic force microscopy(AFM),and variable angle spectroscopy ellipsometry(VASE),respectively.Results show that the sensor performs a good linear relationship in the range of 0.21-10μg/mL for BLG,and the detection of limit is 0.12μg/mL.The sensor could detect the BLG in camel milk powder quickly and sensitively with a recovery rate between 100.2%and 100.7%.

关 键 词：Β-乳球蛋白 多巴胺 分子印迹 SPR传感器 聚(2-甲基-2-噁唑啉) 

分 类 号：O64[理学—物理化学]