云南沙棘果实3种黄酮类化合物的抑菌活性  

Antimicrobial Activity of Three Flavonoids of Hippophae rhamnoides subsp.yunnanensis Berries

作　　者：张金山 杨诗涵 杨红 张秀娟 刘治廷[1,2] 陈小强 ZHANG Jinshan;YANG Shihan;YANG Hong;ZHANG Xiujuan;LIU Zhiting;CHEN Xiaoqiang(Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040,China;College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040,China;College of Resources and Environment,Tibet Agriculture&Animal Husbandry University,Nyingchi 860000,China;Engineering Research Center of Forest Bio-preparation,Ministry of Education,Northeast Forestry University,Harbin 150040,China;Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-based Active Substances,Harbin 150040,China;National Engineering Laboratory of BioResource EcoUtilization,Harbin 150040,China)

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,黑龙江哈尔滨150040 [2]东北林业大学化学化工与资源利用学院,黑龙江哈尔滨150040 [3]西藏农牧学院资源与环境学院,西藏林芝860000 [4]东北林业大学林业生物制剂教育部工程研究中心,黑龙江哈尔滨150040 [5]黑龙江省林业活性物质生态利用重点实验室,黑龙江哈尔滨150040 [6]生物资源生态利用国家地方联合工程实验室,黑龙江哈尔滨150040

出　　处：《食品工业科技》2023年第15期78-84,共7页Science and Technology of Food Industry

基　　金：西藏自治区自然科学基金项目(XZ202101ZR0019G);西藏农牧学院林学学科创新团队建设项目(藏财预指2020-001);中央引导地方区域创新体系建设项目(XZ202201YD0025C)。

摘　　要：研究云南沙棘果实中3种主要黄酮类化合物对4种病原菌的抑制作用。首先采用滤纸片法检测沙棘果实总黄酮对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌、白色念珠菌的抑制效果;其次通过超高效液相色谱-串联质谱(Ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)对总黄酮进行成分鉴定并利用高效液相色谱(High performance liquid chromatography,HPLC)定量检测其主要成分,最后采用二倍稀释法和考马斯亮蓝法评价3种主要黄酮类成分的抑菌效果。结果表明,云南沙棘果实黄酮提取物中主要黄酮类成分为异鼠李素-3-O-葡萄糖-7-O-鼠李糖苷(Isorhamnetin-3-O-glucoside-7-O-rhamnoside,Is-3-G-7-Rh)、芦丁(Quercetin-3-Orutinoside,Qu-3-R)、异鼠李素-3-O-葡萄糖苷(Isorhamnetin-3-O-glucoside,Is-3-G),含量分别为38.43±0.85、29.44±0.42、21.11±0.55 mg/g;总黄酮及3种主要黄酮类成分对4种供试菌均有一定的抑制作用,Is-3-G-7-Rh和Is-3-G对4种菌的抑制效果均优于Qu-3-R和总黄酮,蛋白检测结果表明沙棘黄酮使细胞内蛋白流出导致菌体死亡。本研究可为沙棘属植物果实中黄酮类化合物在抑菌方面的应用提供科学依据。This study aimed to investigate the inhibitory effects of three major flavonoids from the Hippophae rhamnoides subsp.yunnanensis berries on four pathogenic microorganisms.The antimicrobial activity of total flavonoids from Hippophae rhamnoides subsp.yunnanensis berries on Staphylococcus aureus,Escherichia coli,Bacillus subtilis and Candida albicans was firstly measured by filter paper method.Then,the total flavonoids compositions were identified by ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)and the main components were quantified by high performance liquid chromatography(HPLC).Finally,the Bradford method and the double dilution method were used to assess the antimicrobial effect of the three flavonoids.The results showed that the major flavonoid components of the Hippophae rhamnoides subsp.yunnanensis berries were isorhamnetin-3-O-glucoside-7-O-rhamnoside(Is-3-G-7-Rh),quercetin-3-O-rutinoside(Qu-3-R)and isorhamnetin-3-O-glucoside(Is-3-G),which with the contents of 38.43±0.85,29.44±0.42 and 21.11±0.55 mg/g,respectively.The extract and its three main flavonoids had inhibitory effect on the four microorganisms.The inhibitory effects of Is-3-G-7-Rh and Is-3-G on the microorganisms were better than Qu-3-R and extract,and the results of protein detection showed that flavonoids induced antimicrobial death through intracellular protein efflux.This study can provide a scientific basis for the application of flavonoids in the berries of Hippophae rhamnoides for antimicrobial purposes.

关 键 词：云南沙棘 细菌 成分鉴定 抑菌活性 抑菌机理 

分 类 号：TS201.3[轻工技术与工程—食品科学]