卷烟主流烟气有害成分遗传分析  

Genetic Analysis of Harmful Components in Main Stream Cigarette Smoke

作　　者：童治军[1] 唐石云 徐永明 方敦煌[1] 陈学军[1] 冯颖杰 杨宗灿 刘文召 张婷婷 杨金初 肖炳光[1] TONG Zhijun;TANG Shiyun;XU Yongming;FANG Dunhuang;CHEN Xuejun;FENG Yingjie;YANG Zongcan;LIU Wenzhao;ZHANG Tingting;YANG Jinchu;XIAO Bingguang(Yunnan Academy of Tobacco Agricultural Sciences,Key Laboratory of Tobacco Biotechnological Breeding,National Tobacco Genetic Engineering Research Center,Kunming 650021,China;Technology Centre,China Tobacco Yunnan Industrial Co.,Ltd.,Kunming 650231,China;Technology Center,China Tobacco Henan Industrial Co.,Ltd.,Zhengzhou 450000,China)

机构地区：[1]云南省烟草农业科学研究院,烟草行业烟草生物技术育种重点实验室,国家烟草基因工程研究中心,昆明650021 [2]云南中烟工业有限责任公司技术中心,昆明650231 [3]河南中烟工业有限责任公司技术中心,郑州450000

出　　处：《中国烟草科学》2023年第3期16-22,共7页Chinese Tobacco Science

基　　金：中国烟草总公司项目[110201801029(JY-06)、110202101038(JY-15)];中国烟草总公司云南省公司项目(2018530000241005、2022530000241009)。

摘　　要：为深入解析卷烟主流烟气中有害成分的遗传机制,以烤烟Y3和K326为亲本构建的重组自交系群体(RIL)为材料,采用植物数量性状的主基因+多基因混合遗传模型对该群体连续3个世代(F6:7、F7:8和F8:9)的一氧化碳(CO)、氢氰酸(HCN)、4-甲基亚硝胺基-1-3-吡啶基-1-丁酮(NNK)、氨(NH3)、苯并芘{B[a]P}、苯酚(PHE)、巴豆醛(CRO)和焦油(TAR)等8个主流烟气有害成分性状进行遗传分析。结果表明:(1)8个主流烟气有害成分性状在连续3个世代中均呈单峰或多峰的正态或偏正态分布,属典型的数量性状。(2)在连续3个世代中,8个性状的最优遗传模型均为4对部分等加性主基因模型,其中,CO、HCN、NNK、NH3、B[a]P和CRO的最优遗传模型为4MG-EEA;PHE和TAR的最优遗传模型为4MG-EEEA。(3)上述性状在连续3个世代中均受主基因遗传模型控制,主基因遗传率极高(均值为89.01%)且远大于环境(非遗传)因素影响。综上,这些性状主要由遗传基础决定的,可为选育烟草低危害良种提供理论依据。In order to analyze the genetic mechanisms of harmful components in mainstream cigarette smoke,the recombinant inbred lines(RIL)crossed by flue-cured tobaccos Y3 and K326 were used in the major gene plus polygene mixed genetic model method analyses for Carbon Monoxide(CO),Hydrocyanic acid(HCN),4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone(NNK:),Ammonia(NH3),Benzo[a]pyrene(B[a]P),Phenol(PHE),Crotonaldehyde(CRO)and Tar(TAR)in three consecutive generations(2018F6:7,2019F7:8 and 2020F8:9).The main results were as follows:(1)The 8 traits value of RIL populations in three generations all showed normal or partial normal distribution of single or multi peaks,belonging to typical quantitative inheritance.(2)The optimal genetic models for the 8 traits were all controlled by four major genes with partially equally additive(PEA)effects inheritance in three consecutive years.The optimal genetic model of CO,HCN,NNK,NH3,B[a]P and CRO was 4MG-EEA,controlled by four major genes with partially equally additive 1(PEA1)effect mixed inheritance;The optimal genetic model of PHE and TAR was 4MG-EEEA,controlled by four major-genes with partially equally additive 2(PEA2)effect mixed inheritance.(3)These typical harmful components in mainstream cigarette smoke were only controlled by the major gene genetic model in 3 generations,and the heritability of the main genes was higher than 85%(mean 89.01%)and much greater than that of the environmental effect.In conclusion,these traits are mainly determined by genetic basis,which can provide theoretical basis for cultivating low-harm tobacco varieties.

关 键 词：重组自交系群体 主流烟气有害成分 主基因+多基因 遗传分析 

分 类 号：S572.03[农业科学—烟草工业]