地方晒烟琼中五指山-1的青枯病抗性鉴定与指纹图谱开发  

Identification of Bacterial Wilt Resistance and Fingerprint Development of Sun-cured Tobacco Qiongzhongwuzhishan-1

作　　者：王亚男 耿锐梅[1] 李世斌 肖志亮 刘正文 贾振国 杨爱国[1] 任民[1] WANG Yanan;GENG Ruimei;LI Shibin;XIAO Zhiliang;LIU Zhengwen;JIA Zhenguo;YANG Aiguo;REN Min(Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Qingdao 266101,China)

机构地区：[1]中国农业科学院烟草研究所,青岛266101

出　　处：《中国烟草科学》2023年第3期77-84,共8页Chinese Tobacco Science

基　　金：中国烟草总公司烟草基因组计划重大专项[110202201002(JY-02)、110202201008(JY-08)];中国烟草总公司贵州省公司科技项目(2021XM05);中央级科研院所基本科研业务费专项(1610232022008)。

摘　　要：鉴定抗烟草青枯病的优异种质资源,拓宽青枯病抗性品种的遗传背景,提高品种抗病水平,是当前烟草抗性育种的研究重点。以Dixie Bright 101、K326、红花大金元为对照品种,在室内接种FJ-SMNT、FQY-4、Y45等3株菌株,对地方晒烟种质资源琼中五指山-1进行病情指数调查与抗性评价,并基于种质资源重测序数据,开发了琼中五指山-1的特异性SNP指纹图谱。结果表明,琼中五指山-1发病缓慢,病情轻微,病情指数增长平缓,对3株菌株均有良好的抗性,抗性水平显著高于抗病对照。构建的特异性指纹图谱由339个SNP位点组成,基于云计算平台开发了该指纹图谱的检测程序并利用测序技术对指纹图谱的可靠性进行了验证,证明了该指纹图谱可以从随机取样的样品中准确鉴定出琼中五指山-1。琼中五指山-1是目前国内发现的青枯病抗性最好的种质资源之一。The identification of excellent resistance germplasm resources to bacterial wilt,broadening the genetic background and improving the disease resistance level of tobacco varieties are the research focuses of current tobacco resistance breeding.In this study,Dixie Bright 101,K326,and Honghuadajinyuan were used as control varieties,and three Ralstonia solanacearum strains FJ-SMNT,FQY-4,and Y45 were used to inoculate tobacco plants.We then recorded the disease index and evaluated the bacteria wilt resistance of Qiongzhongwuzhishan-1,a local sun-cured tobacco germplasm resource.And based on the resequencing data of germplasm resources,the characteristic SNP fingerprint of Qiongzhongwuzhishan-1 were developed.The results showed that Qiongzhongwuzhishan-1 had a slow onset,mild disease occurence,and a mild increase in the disease index,with good resistance to all three strains.The level of resistance was significantly higher than that of the resistant control.The specific fingerprint consisted of 339 SNP loci,and a detection program for this fingerprint was developed based on the cloud computing platform.The reliability of this fingerprint was verified using sequencing technologies,and it was demonstrated that this fingerprint could accurately identify Qiongzhongwuzhishan-1 from randomly sampled specimens.The results from this study indicated that Qiongzhongwuzhishan-1 is one of the germplasm resources with the best resistance to tobacco bacteria wilt found in China.

关 键 词：青枯病 晒烟 种质资源 抗性鉴定 指纹图谱 

分 类 号：S572.03[农业科学—烟草工业]