HPLC同时测定2,4-二氨基苯氧基乙醇盐酸盐和2-氨基-4-羟乙氨基茴香醚硫酸盐  

Determination of 2,4-diaminophenoxy Ethyl Alcohol Hydrochloride and 2-amino-4-hydroxyethyl Amino Anisyl Ether Sulfateby HPLC

作　　者：王莉 曾浩洋 WANG Li;ZENG Hao-yang(Shaoyang Institute For Food and Drug Control,Hunan Shaoyang 422000,China)

机构地区：[1]邵阳市食品药品检验所,湖南邵阳422000

出　　处：《广州化工》2023年第9期91-93,共3页GuangZhou Chemical Industry

摘　　要：建立了同时测定2,4-二氨基苯氧基乙醇盐酸盐和2-氨基-4-羟乙氨基茴香醚硫酸盐的高效液相色谱方法。样品经用甲醇-5 mmol/L乙酸铵溶液(含亚硫酸氢钠2 g/L)(1∶1)作为流动相,采用C_(18)色谱柱进行分离,以甲醇-5 mmol/L乙酸铵溶液(13∶87)为流动相进行洗脱,检测波长240 nm,流速1.0 mL/min,柱温30℃,结果表明两种染发剂色谱峰分离良好,两种染发剂标准曲线线性系数为0.999,低、中、高三种水平的加标回收率为98.6%~105.5%,相对标准偏差为0.9%~2.3%。该方法简便快速,可用于两种染料的同时测定。A high performance liquid chromatograph method for the simultaneous determination of 2,4-diaminophenoxy ethyl alcohol hydrochloride and 2-amino-4-hydroxyethyl amino anisyl ether sulfate was established.The sample was extracted and separated with methanol-5 mmol/l ammonium acetate solution(containing 2 g/L sodium bisulfite)(1∶1),then separated with C_(18)column and eluted with methanol-5 mmol/L ammonium acetate solution(13∶87)as mobile phase,under the conditions of detection wavelength of 240 nm,flow rate of 1.0 mL/min and column temperature of 30℃.The result showed that the chromatographic peaks of two hair dyes were well separated,the linear coefficients of the standard curves of the two hair dyes were 0.999,the recoveries of the low,middle and high levels were 98.6%~105.5%,and the relative standard deviation was 0.9%~2.3%.The method was simple and rapid,and could be used for simultaneous determination of two dyes.

关 键 词：化妆品 染料 高效液相色谱法 

分 类 号：TQ658.34[化学工程—精细化工]