从腹泻实验兔分离的一株大肠埃希菌的生物学特性研究  

Study on Biological Characteristics of Escherichia Coli Isolated from Experimental Rabbits with Diarrhea

作　　者：董浩 刘志国 李楠 邢进[1] 许中衎 邢壮壮 冯育芳[1] 马丽颖[1] DONG Hao;LIU Zhiguo;LI Nan;XING Jin;XU Zhongkan;XING Zhuangzhuang;FENG Yufang;MA Liying(National Institutes for Food and Drug Control,National Rodent Laboratory Animal Resources Center,Beijing 102629,China)

机构地区：[1]中国食品药品检定研究院国家啮齿类实验动物资源库,北京102629

出　　处：《实验动物科学》2023年第4期49-54,共6页Laboratory Animal Science

摘　　要：目的研究从腹泻实验兔分离到的大肠埃希菌的生物学特性。方法对一株从因腹泻而死亡的SPF级实验兔体内分离的致病性大肠埃希菌的遗传谱系、抗生素敏感性、消毒液敏感性以及细菌携带的耐药基因和毒力基因进行较为全面的研究。结果该大肠埃希菌属于B1型遗传谱系,携带毒力基因eaeA,属于肠致病性大肠埃希菌。对常用的5种消毒液敏感,对于青霉素、氨苄西林、头孢氨苄、头孢拉定、四环素、多西环素、米诺霉素、红霉素、麦迪霉素、万古霉素、复方新诺明等11种抗生素耐药,携带aadA1,gyrA,sul1和tetA等4个抗生素耐药基因。结论从因腹泻而死亡的SPF级实验兔体内分离的致病性大肠埃希菌为一株B1型遗传谱系的肠致病性大肠埃希菌,具有多重耐药性,对常用的消毒剂均敏感。Objective To reveal the biological characteristics of E.coli isolated from experimental rabbits with diarrhea.Method The genetic lineage,antibiotic sensitivity,disinfectant sensitivity,drug resistance gene and virulence related gene of the pathogenic Escherichia coli strain isolated from SPF rabbits died of diarrhea were studied comprehensively.Result The Escherichia coli strain belonged to B1 type genetic lineage,and carried the virulence gene eaeA,which was the classic virulence genes of enteropathogenic Escherichia coli(EPEC).In addition,this isolated strain was sensitive to 5 commonly used disinfectants.Besides,it was resistant to 11 antibiotics including penicillin,ampicillin,cephalexin,cephradine,tetracycline,doxycycline,minocycline,erythromycin,midecamycin,vancomycin and cotrimoxazole,and carried 4 drug resistance genes,including aadA1,gyrA,sul1 and tetA.Conclusion The pathogenic Escherichia coli strain isolated from SPF rabbits died of diarrhea is a type B1 genetic pedigree EPEC with multiple drug resistance and it is sensitive to common disinfectants.

关 键 词：肠致病性大肠埃希菌 耐药性 毒力基因 腹泻 

分 类 号：S855.1[农业科学—临床兽医学]