猪心传导系统的定位及形态学特点  

Localization and morphological characteristics of the porcine cardiac conduction system

作　　者：李慧慧 陈燕娥 李青蔓 康海艺 朱思明 陈起运 张海英[1,2,3] 刘辉 Li Huihui;Chen Yane;Li Qingman;Kang Haiyi;Zhu Siming;Chen Qiyun;Zhang Haiying;Liu Hui(Hainan Provincial Key Laboratory of Tropical Brain Research and Translation,Hainan Medical College,Haikou 570100,China;Human Anatomy Teaching and Research Section,Hainan Medical College,Haikou 570100,China;Science Research Center,Hainan Medical College,Haikou 570100,China;The First Affiliated Hospital,Hainan Medical College,Haikou 570100,China;The Second Affiliated Hospital,Hainan Medical College,Haikou 570100,China)

机构地区：[1]海南省热带脑科学研究与转化重点实验室,海南医学院,海口570100 [2]人体解剖学教研室,海南医学院,海口570100 [3]科学实验中心,海南医学院,海口570100 [4]第一附属医院,海南医学院,海口570100 [5]第二附属医院,海南医学院,海口570100

出　　处：《解剖学杂志》2023年第2期110-114,共5页Chinese Journal of Anatomy

基　　金：国家自然科学基金(81560042)。

摘　　要：目的:通过光镜、荧光显微镜观测猪心传导系统的形态学特点。方法:取新鲜猪心,通过局部解剖学方法明确心传导系统细胞的位置,经Masson染色验证后用心传导细胞的特异性标记物结蛋白作进一步的确认。结果:猪的窦房结和房室结细胞均呈小而不规则的椭圆形,中间有结动脉穿过;房室束细胞呈长条索状,左、右束支细胞形成分散排列的椭圆形细胞束,左束支细胞呈2~3行规律排列于心内膜下,右束支细胞成团分布;浦肯野细胞呈单行空泡状排列于心内膜下。传导细胞周围均有大量纤维结缔组织,结蛋白在这些传导细胞中均有不同程度的表达。结论:经Masson染色明确了猪心的传导系统细胞位置,并且得到了结蛋白的证实,为猪心传导系统的基础研究提供了生物学资料。Objective:This study aims to observe and identify the morphological characteristics of porcine cardiac conduction system using light microscopy and fluorescence microscopy.Methods:The cardiac conduction system was located by topography in fresh porcine hearts and identified using Masson staining,followed by further validation using desmin,a specific marker for cardiac conduction cells.Results:Porcine sinoatrial and atrioventricular cells were characterized as small and irregular spherical,with nodal arteries running through the middle.Atrioventricular bundle cells were elongated and slender,left and right bundle branch cells formed narrow,discrete,rounded groups of fascicles,left bundle branch cells were arranged in 2 to 3 rows beneath the endocardium,and right bundle branch cells were distributed in clusters.Purkinje cells were arranged in the subendocardium in a single line.The conductive cells were surrounded by a large amount of fibrous connective tissue.Desmin was expressed to varying degrees in these transduction cells.Conclusion:The porcine cardiac conduction system cells are confirmed by Masson staining and subsequently also by desmin.This study provides biological data for the fundamental study of the porcine cardiac conduction system.

关 键 词：心传导系统 结蛋白 形态学 MASSON染色 猪 

分 类 号：R322-33[医药卫生—人体解剖和组织胚胎学]