健脾益肾方对痛风性肾损害患者尿液微RNA表达变化的研究  

Study on the changes of urinary microRNA expression of Jianpi Yishen decoction in patients with gout renal damage

作　　者：陈坚翱 罗恒 胡艳艳 徐红[1] CHEN Jian’ao;LUO Heng;HU Yanyan;XU Hong(Department of Science and Education,Hangzhou Hospital of Traditional Chinese Medicine,Hangzhou 310007,Zhejiang,China;Department of Neurology,Hangzhou Fuyang Hospital of Traditional Chinese Medicine,Hangzhou 311400,Zhejiang,China)

机构地区：[1]杭州市中医院科教部,浙江杭州310007 [2]杭州市富阳区中医院神经内科,浙江杭州311400

出　　处：《中国现代医生》2023年第22期80-83,共4页China Modern Doctor

基　　金：浙江省科技计划项目(2017C33196)。

摘　　要：目的分析健脾益肾方干预前后痛风性肾损害患者尿液微RNA(microRNA,miRNA)的表达变化。方法通过高通量测序法检测早期痛风性肾损害患者干预前后尿液外泌体源性miRNA的表达谱,并比较表达变化情况。结果早期痛风性肾损害患者与正常人群的尿液外泌体源性miRNA有明显差异;早期痛风性肾损害患者健脾益肾方中药干预后,19个可作为生物标记物的miRNA中有5个平均表达值上调,14个平均表达值下调。结论健脾益肾方对痛风性肾损害患者的miRNA表达有一定调节作用,差异表达的miRNA可能参与肾损害的发生发展。Objective To analyze the changes of microRNA(miRNA)expression in urine of patients with gout renal damage before and after the intervention of Jianpi Yishen decoction.Methods The expression profiles of urine exosome-derived miRNAs were detected by high-throughput sequencing before and after intervention in patients with early gout renal damage,and the expression changes were compared.Results Urine exosome-derived miRNAs were significantly different between the patients with early gout renal damage and the normal population.After the intervention of Jianpi Yishhen decoction,the expression of 19 miRNAs that could be used as biomarkers were up-regulated in 5 and down-regulated in 14.Conclusion Jianpi Yishen decoction can regulate the expression of miRNA in patients with gout renal damage to some extent,and differentially expressed miRNA may be involved in the occurrence and development of renal damage.

关 键 词：痛风性肾损害 微RNA 健脾益肾方 

分 类 号：R589.7[医药卫生—内分泌]