TLR2、IRF-5基因多态性对新生儿败血症易感性的影响及交互作用  

The impact and interaction of TLR2 and IRF-5 gene polymorphisms on the susceptibility to neonatal sepsis

作　　者：徐征[1] 杜晨 高宇 夏兰兰[1] 李烨 李宁[3] Xu Zheng;Du Chen;Gao Yu;Xia Lanlan;Li Ye;Li Ning(Clinical Laboratory,Baoding Children′s Hospital,Baoding 071000,China;Clinical Laboratory,the 82nd Group Army Hospital,Baoding 071000,China;Department of Neonatal,Baoding Children′s Hospital,Baoding 071000,China)

机构地区：[1]保定市儿童医院检验科,保定071000 [2]中国人民解放军陆军第八十二集团军医院检验科,保定071000 [3]保定市儿童医院新生儿科,保定071000

出　　处：《中国医师杂志》2023年第7期1025-1029,共5页Journal of Chinese Physician

基　　金：河北省保定市计划项目(18ZF246)。

摘　　要：目的探讨Toll样受体2(TLR2)、干扰素调节因子5(IRF-5)基因多态性对新生儿败血症易感性的影响及交互作用。方法前瞻性选取保定市儿童医院2018年7月至2021年8月收治的新生儿败血症患儿78例作为研究组,另选取同期健康新生儿78例作为对照组。比较两组新生儿TLR2、IRF-5基因多态性,不同基因型患儿炎症标志物[C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)]水平,评价TLR2、IRF-5基因型与炎症标志物及新生儿败血症易感性的关系,并分析两者基因多态性对新生儿败血症易感性的交互作用。结果两组新生儿TLR2(rs3804099)、IRF-5(rs2004640)位点基因型分布、等位基因频率差异有统计学意义(均P<0.05);TLR2(rs3804099)位点TT基因型患儿血清CRP、TNF-α、IL-6水平[(111.12±30.87)mg/L、(77.50±20.02)pg/ml、(40.27±11.31)pg/ml]高于CC/CT基因型患儿[(72.46±24.51)mg/L、(54.18±17.65)pg/ml、(28.34±9.05)pg/ml],差异均有统计学意义(均P<0.05)。IRF-5(rs2004640)位点TT基因型患儿血清CRP、TNF-α、IL-6水平[(113.90±28.94)mg/L、(79.84±19.82)pg/ml、(41.05±11.49)pg/ml]高于GG/GT基因型患儿[(70.88±22.16)mg/L、(52.27±16.73)pg/ml、(27.96±9.75)pg/ml],差异均有统计学意义(均P<0.05)。TLR2(rs3804099)位点、IRF-5(rs2004640)位点TT基因型与血清CRP、TNF-α、IL-6水平呈正相关(均P<0.05)。TLR2(rs3804099)位点、IRF-5(rs2004640)位点TT基因型是新生儿败血症易感性的独立危险因素(均P<0.05);TLR2(rs3804099)位点TT基因型与IRF-5(rs2004640)位点TT基因型在新生儿败血症易感性中呈正向交互作用(OR=7.467,γ=1.728)。结论TLR2(rs3804099)位点TT基因型、IRF-5(rs2004640)位点TT基因型会显著增加新生儿败血症易感性,且两者存在正向交互作用。Objective To investigate the impact and interaction of Toll like receptor 2(TLR2)and interferon regulatory factor 5(IRF-5)gene polymorphisms on the susceptibility to neonatal sepsis.Methods A total of 78 cases of neonatal septicemia patients admitted to Baoding Children′s Hospital from July 2018 to August 2021 were prospectively selected as the study group,and 78 cases of healthy newborns in the same period were selected as the control group.The TLR2 and IRF-5 gene polymorphisms and the levels of inflammatory markers[C-reactive protein(CRP),tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)]in different genotypes of infants were compared between the two groups.We evaluated the relationship between TLR2 and IRF-5 genotypes,inflammatory markers,and susceptibility to neonatal sepsis,and analyzed the interaction between their gene polymorphisms and susceptibility to neonatal sepsis.Results There were significant differences in the distribution of TLR2(rs3804099)and IRF-5(rs2004640)loci genotype and Allele frequency between the two groups(all P<0.05);The serum CRP,TNF-α,and IL-6 levels in children with TLR2(rs3804099)genotype TT genotype[(111.12±30.87)mg/L,(77.50±20.02)pg/ml,(40.27±11.31)pg/ml]were higher than those in children with CC/CT genotype[(72.46±24.51)mg/L,(54.18±17.65)pg/ml,(28.34±9.05)pg/ml],and the differences were statistically significant(all P<0.05).The serum CRP,TNF-α,and IL-6 levels[(113.90±28.94)mg/L,TNF-α(79.84±19.82)pg/ml,IL-6(41.05±11.49)pg/ml]in children with the IRF-5(rs2004640)TT genotype were higher than those in children with the GG/GT genotype[(70.88±22.16)mg/L,(52.27±16.73)pg/ml,(27.96±9.75)pg/ml],and the differences were statistically significant(all P<0.05).The TT genotypes at TLR2(rs3804099)and IRF-5(rs2004640)loci were positively correlated with serum CRP,TNF-α,and IL-6 levels(all P<0.05);The TT genotypes at TLR2(rs3804099)and IRF-5(rs2004640)loci were independent risk factors for susceptibility to neonatal sepsis(all P<0.05);The TT genotype at the TLR2(rs3804099)

关 键 词：TOLL样受体2 干扰素调节因子5 多态性 单核苷酸 败血症 新生儿 

分 类 号：R722.131[医药卫生—儿科]