黄蟌属(蜻蜓目:均翅亚目)稚虫形态分类特征稳定性研究  

STABILITY OF DIAGNOSTIC MORPHOLOGICAL CHARACTERS IN CERIAGRION LARVAE (ODONATA: ZYGOPTERA)

作　　者：曾征 陈雪伊 于昕 ZENG Zheng;CHEN Xue-Yi;YU Xin(College of Life Sciences,Chongqing Normal University,Chongqing 401331,China)

机构地区：[1]重庆师范大学生命科学学院,重庆401331

出　　处：《水生生物学报》2023年第10期1659-1668,共10页Acta Hydrobiologica Sinica

基　　金：国家科学技术部《动物志》编研项目(2015FY210300)资助。

摘　　要：基于线粒体基因COI、核基因ITS序列数据,结合成虫、稚虫相互匹配的方法,文章对蜻蜓目广布种长尾黄蟌Ceriagrion fallax Ris和翠胸黄蟌Ceriagrion auranticum Fraser稚虫的尾鳃和上颚这两个传统的形态鉴定特征的稳定性进行了分析,结果显示:(1)长尾黄蟌稚虫尾鳃黑斑的有无、数量、颜色等特征均不稳定,且与种群、性别无关;(2)两种稚虫的上颚齿式特征在同种群内也呈现明显的不稳定性。因此,两种稚虫的尾鳃和上颚特征不稳定,鉴定时应慎用。这暗示蜻蜓目其他物种的形态特征或存在类似的不稳定性。Although dragonfly larvae can be good bioindictors in fresh water environment assessments,their identification based on morphological characteristics is still a tough work,especially at species level.In the present study,116 larvae of two Chinese widespread damselfly,Ceriagrion fallax Ris and Ceriagrion auranticum Fraser,were identified depending on sequence data of the mitochondrial genes Cytochrome Oxidase I(COI)and nuclear ribosomal genes ITS.The stability of two important morphological characters,caudal gills and mandibles,was discussed.For molecular analysis,LCO1490d(TTTCTACWAACCAYAAAGATATTGG)and HCO2198d(TAAACTTCWGGRTGTCC AAARAATCA)were used as COI primers,and Vrain2F(CTTTGTACACACCGCCCGTCGCT)and Vrain2R(TTTCACTCGCCGTTACTAAGGGAATC)as ITS primers.The reaction systems were 31.0μL:DNA template 3.0μL,upstream and downstream primers 2.0μL each,Mix 15.0μL(Jiangsu ComWin Biotech Co.,Ltd’s 2×Es Taq Master Mix),ddH2O 9.0μL.Take 5.0μL PCR amplification products and detect them by electrophoresis using 1.5%agarose gel.The amplification products with detected by electrophoresis,and then sent to Chongqing Tsingke Xingye Biotechnology Co to sequence.Neighbor-Joining(NJ)trees and genetic distance method were using for the molecular identification of larvae,with the help of identified adults’sequences as conference.Relative large size of larval specimens,C.fallax(n=110)and C.auranticum(n=6),were dissected and photographed using LY-WN-OPLENC ultra-clear microphotographic system.Detailed comparative morphological examination was conducted focusing on two typical morphological features,caudal gill and mandibles.Our result showed that:(1)the presence or absence,numbers,and color of the black spots on the caudal gill were unstable,which have nothing to do with populations and sex;(2)characters of mandibles were also variable even within the same population.The discovery of the instability of the two typical diagnostic morphological characters indicates that characters of caudal gills and mandibles,at least,in these tw

关 键 词：形态特征 尾鳃 上颚 稳定性 分子鉴定 蜻蜓稚虫 

分 类 号：Q174[生物学—水生生物学]