Validating field regeneration capacity for selected accessions of Gossypium hirsutum using callus induction and regeneration capacity  

作　　者：TAJO Sani Muhammad PAN Zhaoe HE Shoupu JIA Yinhua MAHMOOD Tahir NAZIR Mian Fasil HU Daowu WANG Liru SADAU Salisu Bello IBRAHIM Sani AUTA Umar GENG Xiaoli DU Xiongming 

机构地区：[1]Zhengzhou Research Base,National Key Laboratory of Cotton Bio‑Breeding and Integrated Utilization,Zhengzhou University,Zhengzhou 450001,China [2]National Key Laboratory of Cotton Bio‑Breeding and Integrated Utilization,Institute of Cotton Research,Chinese Academy of Agriculture Sciences,Anyang,China [3]Oil Crops Reaserch Institute,Chinese Academy of Agriculture Sciences,Wuhan,China [4]Bioresources Development Centre,National Biotechnology Development Agency,Abuja,Nigeria

出　　处：《Journal of Cotton Research》2023年第2期98-111,共14页棉花研究（英文）

基　　金：supported by Ministry of Agriculture and Rural Affairs(19221957).

摘　　要：Background Gossypium hirsutum undergoes rapid clonal propagation to regenerate a mature plant through tissue culture.However,the correlation between cotton leaf regeneration,callus induction,and regeneration ability was still obscure.In this research,cotton leaf regeneration level for 21 accessions in the field(new leaves)was observed after the first harvest,and a comparison between field regeneration level and callus induction with its regeneration capacity(new shoots and roots)for the same 21 accessions was carried out.Agronomic traits,including plant height,leaf area,fresh leaf weight,dry leaf weight,the number of flowers and bolls,and biochemical(proline content)and physiological(chlorophyll and carotenoid content)traits during the flowering stage of 21 upland cotton accessions,were investigated.Result A significant correlation between physiological parameters and callus induction was discovered.Callus induction and regeneration capacity of roots and shoots for hypocotyl,cotyledons,and shoot tip tissues were used to validate field leaf regeneration level after the first harvest.CCRI 24 showed significant leaf regeneration in the field and callus induction capacity through callus induction and regeneration.Conclusion We found a substantial relationship between field regeneration capability and callus induction with its regeneration capacity for the hypocotyl,cotyledons,and shoot tip.The results showed that ZS061,Lumian 378,Jimian 863,and ZS065 have the highest moisture retention capacity,while CCRI 24,Liaoyang Duomaomian,and Beizhe Gongshemian have the lowest moisture retention capacity.CCRI 24 has the highest leaf regeneration capacity in the field,while Beizhe Gongshemian has the lowest leaf regeneration capacity.All our result provides a clue for checking the regeneration capacity through leaf regeneration level in the field.

关 键 词：Upland cotton Agronomic traits Biochemical traits Tissue culture Callus induction Regeneration capacity 

分 类 号：S562[农业科学—作物学]