硫化铼纳米颗粒在能谱CT成像及光热治疗中的应用研究  

Study of rhenium sulfide nanoparticles in spectral CT imaging and photothermal therapy

作　　者：王笑一 王欣宇[2] 张雪宁[3] 李敬 WANG Xiaoyi;WANG Xinyu;ZHANG Xuening;LI Jing(Department of Ultrasound,the Second Hospital of Tianjin Medical University,Tianjin 300211,China;Department of Radiology,Tianjin Key Laboratory of Functional Imaging,Tianjin Medical University General Hospital;Department of Radiology,the Second Hospital of Tianjin Medical University)

机构地区：[1]天津医科大学第二医院超声科,300211 [2]天津医科大学总医院医学影像科 [3]天津医科大学第二医院医学影像科

出　　处：《天津医药》2023年第8期883-887,共5页Tianjin Medical Journal

基　　金：天津市教委科研计划项目(自然科学)(2021KJ229)。

摘　　要：目的探讨硫化铼(ReS2)纳米颗粒的能谱CT成像性能及其在乳腺癌细胞中的光热治疗效果。方法采用一锅法在室温下合成ReS2纳米颗粒;利用X射线光电子能谱、高分辨透射电镜、紫外吸收光谱对ReS2纳米颗粒进行材料学基本性质的表征。比较等效浓度的ReS2纳米颗粒和碘海醇在不同能量下(60~140keV)的能谱CT成像能力。通过测定不同质量浓度的ReS2纳米颗粒水溶液(0、50、100、250和500mg/L)在体外光热升温效果来评估纳米颗粒的光热转换能力。取小鼠乳腺癌细胞4T1与不同质量浓度的ReS2纳米颗粒水溶液(0、20、40、80和100mg/L)共孵育,并通过MTT实验评估纳米颗粒的细胞毒性;通过细胞荧光染色法可视化观察分析ReS2纳米颗粒的光热细胞杀伤效果。结果高分辨透射电镜观察到制备的纳米颗粒粒径<10nm,紫外吸收光谱证实ReS2在808nm处具有较强的吸收能力。当能量在60~140 keV时,等效浓度下ReS2纳米颗粒的CT成像效果明显优于碘海醇溶液。在功率密度为3W/cm2的808nm激光照射下,500mg/LReS2溶液的温度可提高44.90°C±1.2°C,而在相同条件下,纯水的温度仅提高了9.27°C±0.74°C。细胞学实验表明,在无激光照射的情况下,40~100 mg/L的ReS2纳米颗粒处理后,细胞存活率均>85%;在激光照射下,ReS2纳米颗粒浓度>40mg/L时,生存率已<30%。结论ReS2纳米颗粒制备简单,具有良好的生物相容性和光热治疗性能,且能谱CT成像效果显著,极具临床转化潜力。Objective To investigate the spectral CT imaging performance of Rhenium sulfide(ReS2)nanoparticles and their effect on photothermal therapy in breast cancer cells.Methods ReS2 nanoparticles were synthesized by one-pot method at room temperature.The basic materialistic properties of ReS2 nanoparticles were characterized by X-ray photoelectron spectroscopy,high-resolution transmission electron microscopy and UV absorption spectroscopy.The spectral CT imaging capability of ReS2 nanoparticles with the same molar concentration of iodine at different energies(60-140 keV)was compared.The photothermal conversion ability of nanoparticles was evaluated by measuring the in vitro photothermal warming of aqueous solutions of ReS2 nanoparticles with different mass concentrations(0、20、40、80 and 100 mg/L).Mouse breast cancer cells 4T1 were co-incubated with different mass concentrations of ReS2 nanoparticle aqueous solution(0,20,40,80 and 100 mg/L).The cytotoxicity of nanoparticles was evaluated by MTT experiment and cell photothermal killing experiment.The photothermal cell killing effect of ReS2 nanoparticles was visually observed and analyzed by cytofluorescent staining.Results High-resolution transmission electron microscopy observed that the particle size of the prepared nanoparticles was less than 10 nm.The UV absorption spectrum confirmed the strong absorption of ReS2 at 808 nm.When the energy was in the range of 60-140 keV,the CT imaging effect of ReS2 nanoparticles at equivalent molar concentration was significantly better than that of iohexol solution.The temperature of 500 mg/L ReS2 solution could be increased by 44.90°C±1.2°C under 808 nm laser irradiation with a power density of 3 W/cm2,while the temperature of pure water was only increased by 9.27°C±0.74°C under the same conditions.Cellular experiments showed that in the absence of laser irradiation,the survival rate of 4T1 cells treated with ReS2 nanoparticles at concentrations of 40-100 mg/L were all greater than 85%.The cell survival rate was also re

关 键 词：造影剂 纳米粒子 CT成像 硫化铼 光热治疗 

分 类 号：R817.12[医药卫生—影像医学与核医学]