依托咪酯对脂多糖诱导人气道平滑肌细胞炎性损伤的改善作用及其机制  

Improvement of etomidate on inflammatory injury of human airway smooth muscle cells induced by lipopolysaccharide and its mechanism

作　　者：张芸 袁风雷 袁若琳 李钊 ZHANG Yun;YUAN Fenglei;YUAN Ruolin;LI Zhao(Department of Anesthesia,Fengfeng General Hospital of North China Medical and Health Group,Handan 056000,China)

机构地区：[1]华北医疗健康集团峰峰总医院麻醉科,河北邯郸056000

出　　处：《山东医药》2023年第21期30-34,共5页Shandong Medical Journal

基　　金：河北省医学科学研究课题计划项目(20210994)。

摘　　要：目的观察依托咪酯对脂多糖(LPS)诱导人气道平滑肌细胞(ASMCs)炎性损伤的改善作用,并探讨其机制。方法取对数生长期的人ASMCs分为对照组、LPS组、依托咪酯低浓度组、依托咪酯中浓度组、依托咪酯高浓度组。对照组常规培养ASMCs;LPS组加入500 ng/mL的LPS;依托咪酯低浓度组、依托咪酯中浓度组、依托咪酯高浓度组加入500 ng/mL的LPS后,再分别加入5µg/mL、10µg/mL、20µg/mL的依托咪酯。各组细胞继续培养72 h,采用ELISA法检测细胞中炎性因子IL-2、IL-6、TNF-α,采用MTT法测算细胞存活率,采用流式细胞法检测细胞凋亡率,采用荧光定量PCR法检测细胞中转化生长因子(TGF)-β1、Smad家族蛋白3(Smad3)mRNA,采用蛋白印迹法检测细胞中TGF-β1、Smad3蛋白。结果与对照组相比,LPS组、依托咪酯低浓度组、依托咪酯中浓度组、依托咪酯高浓度组细胞中IL-2、IL-6、TNF-α水平均升高(P均<0.05);与LPS组相比,依托咪酯低浓度组、依托咪酯中浓度组、依托咪酯高浓度组细胞中IL-2、IL-6、TNF-α水平均降低(P均<0.05),且依托咪酯中浓度组、依托咪酯高浓度组低于依托咪酯低浓度组(P均<0.05),依托咪酯高浓度组低于依托咪酯中浓度组(P<0.05)。对照组、LPS组、依托咪酯低浓度组、依托咪酯中浓度组、依托咪酯高浓度组细胞存活率分别为100.00%、40.08%±6.04%、52.87%±6.21%、66.48%±7.10%、81.26%±9.54%,组间相比,P均<0.05。对照组、LPS组、依托咪酯低浓度组、依托咪酯中浓度组、依托咪酯高浓度组细胞凋亡率分别为4.40%±1.09%、25.16%±5.07%、19.77%±4.03%、14.71%±2.25%、10.05%±2.05%,组间相比,P均<0.05。与对照组相比,LPS组、依托咪酯低浓度组、依托咪酯中浓度组、依托咪酯高浓度组细胞中TGF-β1 mRNA和蛋白相对表达量、Smad3 mRNA和蛋白相对表达量均升高(P均<0.05);与LPS组相比,依托咪酯低浓度组、依托咪酯中浓度组、依托咪酯高浓度组细�Objective To observe the ameliorative effect of etomidate on lipopolysaccharide(LPS)-induced inflammatory injury of human airway smooth muscle cells(ASMCs)and to explore its mechanism.Methods Human ASMCs in logarithmic growth period were divided into the control group,LPS group,low-concentration etomidate group,medium-concentration etomidate group,and high concentration etomidate group.ASMCs were routinely cultured in the control group.We added 500 ng/mL of LPS to the LPS group,and added 500 ng/mL LPS to the low-concentration etomidate group,medium-concentration etomidate group,high-concentration etomidate group,and then,we added 5,10,and 20μg/mL of etomidate,respectively.The cells in each group were cultured for 72 h.The inflammatory factors IL-2,IL-6,and TNF-αin cells were detected by ELISA,the cell survival rate was measured by MTT,and the apoptosis rate was detected by flow cytometry,the mRNA of transforming growth factor(TGF)-β1 and Smad family protein 3(Smad3)were detected by fluorescence quantitative PCR,and the proteins of TGF-β1 and Smad3 were detected by Western blotting.Results Compared with the control group,the levels of IL-2,IL-6,and TNF-αin the LPS group,low-concentration,medium-concentration,and high-concentration etomidate groups increased(all P<0.05).Compared with the LPS group,the levels of IL-2,IL-6 and TNF-αin the low-concentration,medium-concentration,and high-concentration etomidate groups all decreased(all P<0.05),and those in the medium-concentration etomidate group and high-concentration etomidate group were lower than those in the low-concentration etomidate group(all P<0.05),and those in the high-concentration etomidate group were lower than those of the medium-concentration etomidate group(all P<0.05).The cell survival rates of the control group,LPS group,low-concentration,medium-concentration,and high-concentration etomidate groups were 100.00%,40.08%±6.04%,52.87%±6.21%,66.48%±7.10%,and 81.26%±9.54%,respectively,with statistically significant differences between groups(all

关 键 词：依托咪酯 人气道平滑肌细胞 细胞炎性损伤 细胞存活 细胞凋亡 转化生长因子-β1 Smad家族蛋白3 

分 类 号：R562.25[医药卫生—呼吸系统]