白蜡窄吉丁视觉基因干扰对其颜色敏感性的影响  

Effect of opsin gene interference on color sensitivity of Agrilus planipennis Fairmaire

作　　者：樊智智 张荣 张珣 张真[1] 孔祥波[1] 张苏芳 FAN Zhizhi;ZHANG Rong;ZHANG Xun;ZHANG Zhen;KONG Xiangbo;ZHANG Sufang(Key Laboratory of Forest Protection of National Forestry and Grassland Administration,Ecology and Nature Conservation Institute,Chinese Academy of Forestry,Beijing 100091,China)

机构地区：[1]中国林业科学研究院森林生态环境与自然保护研究所,国家林业和草原局森林保护学重点实验室,北京100091

出　　处：《中国森林病虫》2023年第4期32-35,共4页Forest Pest and Disease

基　　金：中国林业科学研究院基本科研业务费专项“重要林业害虫RNA干扰效率限速机制研究”(CAFYBB2020QC001);国家重点研发计划项目“森林生态系统重要生物危害因子综合防控关键技术研究”(2018YFC1200400)。

摘　　要：白蜡窄吉丁Agrilus planipennis Fairmaire是一种国际检疫性害虫。为弄清视觉基因RNA干扰对白蜡窄吉丁成虫的视觉行为有何影响,基于RNAi及行为测定,对视蛋白干扰后的白蜡窄吉丁进行了颜色敏感性测试。结果显示:正常的白蜡窄吉丁偏好紫色和绿色色板,干扰重要视蛋白基因opsin LW1或者opsin UV2后,选择紫色和绿色色板的成虫数量降低到40%以下;其中干扰opsin LW1显著影响白蜡窄吉丁对绿色的偏好,干扰opsin UV2显著影响对紫色的偏好。从行为层面证明了视蛋白基因干扰可影响白蜡窄吉丁的颜色敏感性,为开发基于视觉干扰的白蜡窄吉丁防控相关产品提供理论参考。Agrilus planipennis is an international quarantine pest.To clarify how opsin genes RNAi affects the visual behavior of the adult A.planipennis,color sensitivity of A.planipennis after opsin genes silence was tested based on behavior observation.The results indicated that A.planipennis preferred purple and green color plates,and the number of adults who chose purple and green color plates decreased to less than 40%treatment after dsRNA injection of opsin LW1 or opsin UV2.In detail,opsin LW1 RNAi influenced green preference,and opsin UV2 RNAi influenced purple preference.Opsin genes RNAi can affect the color sensitivity of A.planipennis obviously on behavior level,and the conclusion will support developing visual interference related products for population control of A.planipennis.

关 键 词：白蜡窄吉丁 视蛋白 RNA干扰 颜色敏感 色板 

分 类 号：S763.38[农业科学—森林保护学]