The transcriptional regulator JAZ8 interacts with the C2 protein from geminiviruses and limits the geminiviral infection in Arabidopsis  

作　　者：Tabata Rosas-Diaz Pepe Cana-Quijada Mengshi Wu Du Hui Gemma Fernandez-Barbero Alberto PMacho Roberto Solano Araceli GCastillo Xiao-Wei Wang Rosa Lozano-Duran Eduardo R.Bejarano 

机构地区：[1]Departamento de Biología Celular,Genética y Fisiología,Facultad de Ciencias,Instituto de Hortofruticultura Subtropical y Mediterránea“La Mayora”(IHSM‐UMA‐CSIC),Universidad de Málaga,Málaga,Spain [2]Shanghai Center for Plant Stress Biology,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 201602,China [3]Institute of Insect Sciences,Zhejiang University,Hangzhou 310058,China [4]Departamento de Genética Molecular de Plantas,Centro Nacional de Biotecnología‐Consejo Superior de Investigaciones Científicas,Madrid 28049,Spain [5]Department of Plant Biochemistry,Centre for Plant Molecular Biology,Eberhard Karls University,Tübingen D‐72076,Germany

出　　处：《Journal of Integrative Plant Biology》2023年第7期1826-1840,共15页植物学报（英文版）

基　　金：supported by a President's International Fellowship Initiative (PIFI) postdoctoral fel owship (No. 2016PB042) from the Chinese Academy of Sciences;the “Programa Juan de la Cierva” (IJCI-2017-33367) from the MCIN and FEDER program UMA20-FEDERJA-132 by AEI and by “ERDF A way of making Europe,” by the “European Union”;Funding for Open Access charge: Universidad de Málaga / CBUA。

摘　　要：Jasmonates(JAs) are phytohormones that finely regulate critical biological processes, including plant development and defense. JASMONATE ZIM-DOMAIN(JAZ) proteins are crucial transcriptional regulators that keep JA-responsive genes in a repressed state. In the presence of JA-Ile, JAZ repressors are ubiquitinated and targeted for degradation by the ubiquitin/proteasome system,allowing the activation of downstream transcription factors and, consequently, the induction of JA-responsive genes. A growing body of evidence has shown that JA signaling is crucial in defending against plant viruses and their insect vectors. Here, we describe the interaction of C2proteins from two tomato-infecting geminiviruses from the genus Begomovirus, tomato yellow leaf curl virus(TYLCV) and tomato yellow curl Sardinia virus(TYLCSaV), with the transcriptional repressor JAZ8 from Arabidopsis thaliana and its closest orthologue in tomato, SlJAZ9. Both JAZ and C2proteins colocalize in the nucleus, forming discrete nuclear speckles. Overexpression of JAZ8did not lead to altered responses to TYLCV infection in Arabidopsis;however, knock-down of JAZ8 favors geminiviral infection. Low levels of JAZ8 likely affect the viral infection specifically,since JAZ8-silenced plants neither display obvious developmental phenotypes nor present differences in their interaction with the viral insect vector. In summary, our results show that the geminivirus-encoded C2 interacts with JAZ8 in the nucleus, and suggest that this plant protein exerts an anti-geminiviral effect.

关 键 词：C2 protein GEMINIVIRUS JASMONATES JAZ8 transcriptional repressor TYLCV 

分 类 号：S432.41[农业科学—植物病理学]