根际NaCl浓度对冰菜形态和生理的影响  

Effects of Rhizosphere NaCl Concentration on Morphology and Physiology of Mesembryanthemum crystallinum L.

作　　者：付婷婷 王昊[1] 赵颖 王蔚兰 王旭[1] FU Tingting;WANG Hao;ZHAO Ying;WANG Weilan;WANG Xu(School of Horticulture,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学园艺学院,海南海口570228

出　　处：《广东农业科学》2023年第6期36-43,共8页Guangdong Agricultural Sciences

基　　金：海南省重点研发计划项目(ZDYF2022SHFZ061);海南省自然科学基金(321RC1032)。

摘　　要：【目的】探究根际NaCl浓度对冰菜的形态和生理的影响,找到适合植物工厂冰菜营养液栽培的适宜NaCl浓度,为冰菜植物工厂生产管理提供参考。【方法】以水晶冰菜种子为材料,选用日本园试营养液配方,在人工光下水培,以未添加NaCl处理为对照(CK),添加NaCl至终浓度分别为3、6、9、12 g/L(分别记为T1、T2、T3、T4)。处理21 d后,分析不同NaCl浓度营养液中冰菜的形态和生理指标,统计表皮盐囊泡面积占比和表皮盐囊泡个数,分析不同NaCl浓度与冰菜形态和生理的关系。【结果】从形态指标与质量来看,冰菜在3 g/L NaCl浓度营养液中,株高、茎粗、叶面积、地下部鲜质量和地上部鲜质量与CK相比无极显著差异;冰菜在6 g/L NaCl浓度营养液中株高和地下部鲜质量和地上部鲜质量与CK相比显著增高,该浓度对冰菜生长有一定的促进作用;冰菜在9、12 g/L NaCl浓度营养液中株高、茎粗、叶面积、地上部鲜质量呈下降趋势,与CK差异达到极显著水平,表明冰菜在9 g/L和12 g/L NaCl浓度营养液中生长受到抑制。从生理指标方面来看,冰菜的可溶性蛋白含量、可溶性糖含量和叶绿素SPAD值随NaCl浓度升高呈上升趋势,在12 g/L NaCl浓度营养液中可溶性蛋白含量、可溶性糖含量和叶绿素SPAD值最高,分别比CK增加50.2%、119.5%和77.9%;从表皮盐囊泡指标来看,冰菜表皮盐囊泡面积占比与表皮盐囊泡个数随营养液中NaCl浓度的升高而增大,在12 g/L NaCl浓度营养液中达到最大,分别比CK增加54.9%、182.1%。【结论】营养液中3~6 g/L NaCl浓度最适于植物工厂冰菜栽培。【Objective】This study aimed to investigate the effects of rhizospheric NaCl concentration on the morphology and physiology of Mesembryanthemum crystallinum L.and determine the suitable NaCl concentration for it’s hydroponic cultivation in plant factories,providing references for production management.【Method】Crystal M.crystallinum L.seeds were used as material,and the Japanese garden general formula was selected for cultivation in nutrient solution under artificial light.With no NaCl added as the control(CK),NaCl was added to the final concentration of 3 g/L(T1),6 g/L(T2),9 g/L(T3),12 g/L(T4),respectively.After 21 days of NaCl treatment,the morphological and physiological indicators were analyzed,and the proportion of epidermal salt vacuole area and the number of epidermal salt vacuoles were counted,so as to analyze the relationship between NaCl concentration and the morphology and physiology of M.crystallinum L..【Result】From the perspective of morphological indicators and biomass,there were no significant differences in plant height,stem thickness,leaf area,root fresh weight and shoot fresh weight compared to the CK when M.crystallinum L.was grown in a nutrient solution with a NaCl concentration of 3 g/L.In the nutrient solution with a NaCl concentration of 6 g/L,M.crystallinum L.showed significantly increased in plant height,root fresh weight and shoot fresh weight compared to the CK,indicating a certain promotion effect on the growth of M.crystallinum L..However,in the nutrient solutions with NaCl concentrations of 9 g/L and 12 g/L,M.crystallinum L.exhibited a decreasing trend in plant height,stem thickness,leaf area,and shoot fresh weight with significant differences compared to the CK.This suggests that the growth of M.crystallinum L.is inhibited in nutrient solutions with NaCl concentrations of 9 g/L and 12 g/L.In terms of physiological indicators,the protein content,soluble sugar content,and chlorophyll SPAD value of M.crystallinum L.increased with increasing NaCl concentration,reaching the h

关 键 词：冰菜 盐分浓度 形态 质量 生理 表皮盐囊泡 耐盐性 

分 类 号：S636.9[农业科学—蔬菜学]