甘蓝型油菜RPD3/HDA1基因家族鉴定及早熟相关基因挖掘  

Identification of RPD3/HDA1 Gene Family in Brassica napus and Mining of Early Maturity Related Members

作　　者：张玉玲 张超 蒋唤唤 梁峰豪 肖华贵[1] 曾兵[1,2] Yuling;ZHANG Chao;JIANG Huanhuan;LIANG Fenghao;XIAO Huagui;ZENG Bing(Oil Research Institute of Guizhou Province,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;Guizhou Jinrui Agricultural Science and Technology Limited Company,Guiyang 550006,China)

机构地区：[1]贵州省农业科学院贵州省油料研究所,贵阳550006 [2]贵州金瑞农业科技有限公司,贵阳550006

出　　处：《西北植物学报》2023年第6期901-911,共11页Acta Botanica Boreali-Occidentalia Sinica

基　　金：早熟杂交油菜新品种黔油早2号中试与示范项目(黔科合成果[2020]1Y052);贵州山区特色油料资源利用研究及实验平台建设项目(黔科中引地[2020]4012号);贵州省油菜产业技术体系项目(GZCYTX2018-08);油菜优质高产宜机收新品种选育项目(黔科合支撑[2022]重点031)。

摘　　要：该研究运用生物信息学方法鉴定甘蓝型油菜RPD3/HDA1基因家族,检测了其在‘黔油早2号’和‘中双11’中的表达水平及2个品种在低温(4℃)和ABA胁迫下该家族基因的表达特征,以探讨RPD3/HDA1基因在甘蓝型油菜中的潜在功能,为早熟油菜抗逆性遗传改良提供理论基础和候选基因。结果表明:(1)在甘蓝型油菜全基因组中共鉴定到28个RPD3/HDA1基因,将其命名为BnHDA1~BnHDA28,聚类为4个亚家族,同一亚家族成员的基因结构较为相似;在该基因家族中共检测到16对复制基因,均为片段重复。(2)顺式作用元件预测统计中共发现675个元件与植物激素、环境胁迫和光响应有关。(3)qRT-PCR分析显示,RPD3/HDA1基因在‘黔油早2号’中的表达量均高于‘中双11’;低温胁迫下,‘黔油早2号’和‘中双11’中RPD3/HDA1基因呈差异表达,与‘中双11’相比,RPD3/HDA1基因在‘黔油早2号’中的下调幅度较大;ABA处理后,RPD3/HDA1基因在2个品种中表达模式不一致,‘黔油早2号’中大部分RPD3/HDA1基因表达量较‘中双11’下调幅度小。研究认为,RPD3/HDA1基因可能在油菜开花中发挥调节作用,而且可能通过激素信号通路和防御信号通路参与油菜的生长发育和防御反应的调节。RPD3/HDA1 gene family plays an important role in plant flowering and stress response.In this study,we used bioinformatics methods to identify the RPD3/HDA1 gene family in Brassica napus,and detected the expression level of this family gene in‘Qianyouzao 2’and‘Zhongshuang 11’,and the gene expression characteristics of the family in two varieties under low temperature(4℃)and ABA stress,in order to explore the potential function of RPD3/HDA1 genes in Brassica napus,and provide theoretical basis and candidate genes for genetic improvement of stress resistance in early maturing rapeseed.The results showed that:(1)A total of 28 RPD3/HDA1 genes were identified in the whole genome of Brassica napus,and they were named BnHDA1-BnHDA28 with clustering into 4 subfamilies,the gene structures of members of the same subfamily were relatively similar,and a total of 16 pairs of replicated genes were detected in this gene family,all of them were fragment repeats.(2)A total of 675 cis-acting elements were found to be related to plant hormones,environmental stress,and light response.(3)qRT-PCR analysis showed that the expression of RPD3/HDA1 genes in‘Qianyouzao 2’was higher than that in‘Zhongshuang 11’.Under low temperature stress,the RPD3/HDA1 genes in‘Qianyouzao 2’and‘Zhongshuang 11’showed differential expression,and compared with‘Zhongshuang 11’,the down-regulation of RPD3/HDA1 genes in‘Qianyouzao 2’was greater.After ABA treatment,the expression pattern of RPD3/HDA1 genes in the two varieties was inconsistent,and the expression level of most RPD3/HDA1 genes in‘Qianyouzao 2’was lower than that in‘Zhongshuang 11’.Research suggested that RPD3/HDA1 genes may play a regulatory role in rape flowering,and may participate in the regulation of rape growth and development and defense response through hormone signaling pathway and defense signaling pathway.

关 键 词：甘蓝型油菜 RPD3/HDA1家族 早熟 胁迫 表达分析 

分 类 号：Q943.2[生物学—植物学] Q789[农业科学—作物学] S565.4