3个玉兰材料对水涝胁迫的响应与耐涝性评价  

Response to Waterlogging Stress and Evaluation of Waterlogging Tolerance of Three Yulania Materials

作　　者：叶康 陈伯祥 杜习武 秦俊 曾丽[3] 胡永红[1,2] YE Kang;CHEN Boxiang;DU Xiwu;QIN Jun;ZENG Li;HU Yonghong(Shanghai Chenshan Botanical Garden,Shanghai 201602,China;Shanghai Engineering Research Center of Urban Tree Ecology and Applications,Shanghai 200020,China;School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China)

机构地区：[1]上海辰山植物园,上海201602 [2]上海城市树木生态应用工程技术研究中心,上海200020 [3]上海交通大学农业与生物学院,上海200240

出　　处：《西北植物学报》2023年第6期940-947,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：上海市绿化和市容管理局科技项目(G212409,G192401);上海市科委科技创新行动计划项目(19DZ1203500)。

摘　　要：为评价星花玉兰及其实生选育品种‘菊花’以及其与柳叶玉兰的杂交品种‘皮鲁埃特’的耐涝性强弱,并为后续星花玉兰耐涝品种选育提供理论依据。通过14 d的水涝胁迫,研究3种玉兰叶片生理、内源激素和光合特性等相关的14个指标变化。利用主成分分析法,比较超氧化物歧化酶(SOD)、丙二醛(MDA)、可溶性蛋白、脱落酸(ABA)和1-氨基环丙烷-1-羧酸(ACC)这5个作为评价耐涝性指标的重要性以及评价耐涝性,同时利用隶属函数分析法对5个指标进行耐涝性评价。结果表明:(1)3种玉兰在水涝胁迫后叶片的净光合速率(P n)、气孔导度(G_(s))、蒸腾速率(T_(r))、叶绿素含量、初始荧光(F_(o))、最大荧光(F_(m))、PSⅡ最大光化学量子产量(F_(v)/F_(m))、PSⅡ有效光化学量子产量(Yield)和可溶性蛋白含量均显著降低,MDA、ABA含量和SOD活性均有不同的增加幅度。‘菊花’和星花玉兰处理组的C_(i)、ACC显著增加,而‘皮鲁埃特’的显著降低。(2)评价3种玉兰耐涝性的生理及内源激素指标重要性依次为ACC、SOD、ABA、可溶性蛋白和MDA。(3)通过主成分分析法和隶属函数分析法对3种玉兰进行综合评价,耐涝性强弱依次为‘皮鲁埃特’、星花玉兰和‘菊花’。The authors evaluated the waterlogging tolerance of Yulania stellata and its seedling breeding cultivar Y.stellata‘Chrysanthemiflora’and its hybrid cultivar Y.‘Piroutte’with Y.salicifolia,to provide theoretical basis for subsequent selection of Y.stellata waterlogging tolerant cultivars.Through 14 days of waterlogging stress,the changes of 14 indexes related to leaf physiology,endogenous hormones and photosynthetic characteristics of three Yulania materials were studied.Using principal component analysis,the importance of superoxide dismutase(SOD),malondialdehyde(MDA),soluble protein,abscisic acid(ABA)and 1-aminocyclopropane-1-carboxylic acid(ACC)as indicators of waterlogging tolerance was compared and waterlogging tolerance was evaluated.The waterlogging tolerance of the five indicators was evaluated by the membership function analysis method.The results showed that:(1)The contents of P n,G_(s),T_(r),chlorophyll,origin fluorescence,maximum fluorescence,PSⅡmaximum photochemical quantum yield,PSⅡeffective photochemical quantum yield and soluble protein in the leaves of the three Yulania materials were significantly decreased after waterlogging stress,and the contents of MDA,ABA and SOD activity were increased by different degrees.However,the intercellular CO_(2)concentration and ACC of Y.stellata‘Chrysanthemiflora’and Y.stellata treatment groups increased significantly,while the C_(i)and ACC of Y.‘Piroutte’decreased significantly.(2)The importance of physiological and endogenous hormone indicators to evaluate the waterlogging tolerance of three Yulania materials was in order:ACC,SOD,ABA,soluble protein,and MDA.(3)According to the comprehensive evaluation of the three cultivars by principal component analysis and membership function analysis,the waterlogging tolerance of the three Yulania materials was in the order of Y.‘Piroutte’,Y.stellata and Y.stellata‘Chrysanthemiflora’.

关 键 词：星花玉兰 耐涝性 主成分分析法 隶属函数分析法 

分 类 号：S685[农业科学—观赏园艺]