多聚腺嘌呤DNA探针及其生物传感应用  

Poly-adenine-based DNA Probes and Their Applications in Biosensors

作　　者：李兰英[1] 陶晴 闻艳丽[1] 王乐乐[1] 郭瑞妍 刘刚[1] 左小磊 Li Lanying;Tao Qing;Wen Yanli;Wang Lelea;Guo Ruiyana;Liu Gang;Zuo Xiaolei(Key Laboratory of Bioanalysis and Metrology for State Market Regulation,Shanghai Institute of Measurement and Testing Technology,Shanghai 201203,China;Institute of Molecular Medicine,School of Medicine,Shanghai Jiao Tong University,Shanghai 200127,China)

机构地区：[1]上海市计量测试技术研究院、国家市场监督管理总局生物分析计量溯源重点实验室,上海201203 [2]上海交通大学医学院分子医学研究院,上海200127

出　　处：《化学学报》2023年第6期681-690,共10页Acta Chimica Sinica

基　　金：国家质量基础设施体系专项(2021YFF0600705);国家自然科学基金(22074093);上海市青年科技英才扬帆计划(21YF1459500)资助.

摘　　要：与传统的巯基DNA探针相比,多聚腺嘌呤DNA探针(polyA DNA探针)无需特别化学修饰,具有合成简单、成本低、稳定性好等优点,利用自身polyA嵌段与金的相互作用即可达到捕获探针在金表面的紧凑和有序自组装的目的,实现了识别和探针固定功能的一体化,在生物传感领域受到广泛关注.本综述首先介绍了polyA DNA探针与金相互作用机理研究,随后分别介绍了polyA DNA探针在比色、荧光、表面增强拉曼散射和电化学生物传感中的研究进展,并探讨了polyA DNA探针在生物传感领域面临的机遇和挑战,以期为食品安全、环境监控、生物医学等领域的进一步发展提供参考.Compared to traditional thiolated DNA probes,poly-adenine-based DNA probes(polyA DNA probes)are free of special chemical modifications,and thus exhibit unique advantages of easy synthesis,low economic cost,and excellent stability.Using their intrinsic polyA fragments,polyA DNA probes are combined onto the gold surface to form a compact and ordered monolayer with both anchoring and recognition capabilities.As a result,polyA DNA probes have attracted numerous research interests in biosensing.In this review,we first presented the mechanism of interaction between polyA DNA probes and the gold surface and then reviewed the applications of the polyA DNA probes in the development of biosensors,including colorimetric biosensors,fluorescence biosensors,surface-enhanced Raman scattering(SERS)biosensors,and electrochemical biosensors.We concluded with a discussion of the opportunities and challenges for polyA DNA probes and expected this review to be informative for the development of biosensors in food safety,environmental monitoring,and biomedicine.

关 键 词：多聚腺嘌呤DNA探针 自组装 生物传感器 生物标志物 

分 类 号：O657[理学—分析化学] TP212.3[理学—化学]