Time-resolved luminescent nanoprobes based on lanthanide nucleotide self-assemblies for alkaline phosphatase detection  

作　　者：Yunqin Zhang Yang Cao Yan Liu Yingjie Yang Mingmao Chen Hang Gao Lushan Lin Xueyuan Chen 

机构地区：[1]CAS Key Laboratory of Design and Assembly of Functional Nanostructures,Fujian Key Laboratory of Nanomaterials,and State Key Laboratory of Structural Chemistry,Fujian Institute of Research on the Structure of Matter,Chinese Academy of Sciences,Fuzhou 350002,China [2]Fujian Science&Technology Innovation Laboratory for Optoelectronic Information of China,Fuzhou 350108,China [3]College of Biological Science and Engineering,Fuzhou University,Fuzhou 350108,China [4]College of Chemistry and Materials Science,Fujian Normal University,Fuzhou 350117,China [5]Fujian College,University of Chinese Academy of Sciences,Fuzhou 350002,China

出　　处：《Nano Research》2023年第8期11250-11258,共9页纳米研究（英文版）

基　　金：supported by the National Key R&D Program of China(No.2022YFB3503700);the National Natural Science Foundation of China(NSFC)(Nos.22135008,U22A20398,and 22275190);the Natural Science Foundation of Fujian Province(Nos.2021L3024,2021Y0067);the Open Fund of State Key Laboratory of Structural Chemistry(No.20210023).

摘　　要：Currently,enzyme-responsive nanomaterials have shown great promise in prognosis or diagnosis of disease biomarker.However,the great obstacle for conventional enzyme-responsive nanomaterials frequently lies in autofluorescence interference,poor monodispersity,uncontrollable size and morphology,low optical stability,and biotoxicity,which fundamentally impede their practical application in biological systems.To overcome these deficiencies,we proposed a novel strategy for reliable and precise detection of an enzyme disease biomarker,alkaline phosphatase(ALP),through lanthanide(Ln^(3+))nucleotide nanoparticles(LNNPs)with extremely improved monodispersity and uniformity,which were achieved by the coordination self-assembly between ATP and Ln^(3+)inside micellar nanoreactor.Specifically,for ATP-Ce/Tb LNNPs,highly improved photoluminescence(PL)emission of Tb^(3+)can be achieved via efficient Ce^(3+)sensitization.We demonstrated that ALP could specifically cleave the phosphorus–oxygen(P–O)bonds of ATP and result in the collapse of ATP-Ce/Tb scaffold,finally leading to the PL quenching of Tb^(3+).By taking advantage of time-resolved(TR)PL technique,the fabricated ATP-Ce/Tb LNNPs presented superior selectivity and sensitivity for the ALP bioassay in complicated serum samples,thus revealing the great potential of ATP-Ce/Tb LNNPs in the areas of ALP-related disease prognosis and diagnosis.

关 键 词：alkaline phosphatase(ALP)-responsive micellar nanoreactor coordination self-assembly lanthanide sensitization time-resolved photoluminescence(TRPL) 

分 类 号：O62[理学—有机化学]