A quadruplex immunochromatographic assay for the ultrasensitive detection of 11 anesthetics  

作　　者：Xianlu Lei Xinxin Xu Li Wang Wei Zhou Liqiang Liu Liguang Xu Hua Kuang Chuanlai Xu 

机构地区：[1]State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China [2]International Joint Research Laboratory for Biointerface and Biodetection,and School of Food Science and Technology,Jiangnan University,Wuxi 214122,China [3]Jiangsu Product Quality Testing and Inspection Institute,Nanjing 210000,China

出　　处：《Nano Research》2023年第8期11269-11277,共9页纳米研究（英文版）

基　　金：This work is financially supported by the National Key Research and Development Program of China(No.2022YFF1101002);Jiangsu Association for Science and Technology Youth Science and Technology Talent Support Project(No.TJ-2021-049).

摘　　要：Anesthetic residues in fish represent a potential risk to human health.Therefore,it is important to develop a sensitive and broad-specific method for the detection of anesthetics.In this study,we developed a colloidal gold-based quadruplex immunochromatographic(Qua-ICS)assay using four highly sensitive monoclonal antibody immunotherapy(mAbs)that simultaneously detected 11 anesthetic residues in fish within 10 min.The colorimetric and cut-off values(COVs)for procaines,eugenols,bupivacaines,and tricaine(TMS)were 0.37–1.1 and 3.3–10,11–222 and 100–2000,0.37 and 3.3,and 111 and 10,000µg/kg,respectively.Quantitative analysis was achieved with a portable strip-reader,and the detection ranges were 0.15–2.6,6.3–677,0.13–2.8,and 83–1245µg/kg for procaines,eugenols,bupivacaines,and tricaine,respectively.Our developed method was reliable and accurate according to the recovery test results and analyses of real samples.Therefore,the strip can be used as an alternative method for the rapid detection of anesthetic residues in fish.

关 键 词：BUPIVACAINE colloidal gold EUGENOL PROCAINE tricaine(TMS) 

分 类 号：O657.7[理学—分析化学]