骨碎补内生菌Lecanicillium sp.GZWMJZ-847中3个新3-氢化萘特特拉姆酸衍生物  

Three new 3-decalinoyltetramic acids produced by endophyte Lecanicillium sp.GZWMJZ-847 from Drynariae Rhizoma

作　　者：陈旭丽 王立平 吴耽 何文文 左明星 王冬阳 朱伟明 CHEN Xu-li;WANG Li-ping;WU Dan;HE Wen-wen;ZUO Ming-xing;WANG Dong-yang;ZHU Wei-ming(State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550014,China;School of PharmaceuticalSciences,Guizhou Medical University,Guiyang 550025,China;Natural Product Research Center of Guizhou Province,Guiyang 550014,China)

机构地区：[1]省部共建药用植物功效与利用国家重点实验室,贵州医科大学,贵州贵阳550014 [2]贵州医科大学药学院,贵州贵阳550025 [3]贵州省天然产物研究中心,贵州贵阳550014

出　　处：《中草药》2023年第13期4096-4103,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(U1812403);贵州省科技计划项目(黔科中引地[2022]4015);贵州省“百人领军人才”计划(朱伟明);博士引进启动基金(天产自J字[2022]02号)。

摘　　要：目的研究骨碎补内生蜡蚧菌Lecanicillium sp.GZWMJZ-847的次级代谢产物和抑菌活性。方法采用大米培养基对菌株Lecanicillium sp.GZWMJZ-847发酵;采用凝胶柱色谱、正反相硅胶色谱等方法对化合物进行分离纯化;采用核磁共振谱、质谱、NMR计算等方法确定化合物的化学结构;采用营养肉汤稀释法测试化合物对病原细菌及酵母菌的抑菌活性、平板法测试化合物对病原霉菌的抑菌活性。结果从菌株的发酵产物中分离鉴定了3个新的和3个已知的3-氢化萘特特拉姆酸衍生物,包括蜡蚧特特拉姆酸A(1)、蜡蚧特特拉姆酸B(2)、蜡蚧特特拉姆酸C(3)、Sch 210971(4)、Sch 210972(5)、Sch 213766(6)。化合物1和4~6对4株人体革兰阳性病原菌和1株植物革兰阴性病原菌具有抑制作用,最小抑菌浓度(minimun inhibitory concentration,MIC)为7.8~125μmol/L。化合物6对油菜菌核病菌和小麦赤霉病菌表现出一定的抑制作用,半数抑制浓度分别为(22.05±0.62)、(55.54±1.72)μg/mL。结论蜡蚧特特拉姆酸A~C(1~3)为新的3-氢化萘特特拉姆酸衍生物,骨碎补内生菌Lecanicillium sp.GZWMJZ-847产生的3-氢化萘特特拉姆酸对人体病原菌和植物病原菌可表现出抑制作用。Objective To investigate the secondary metabolites and its anti-microbial activities of the endophytic fungus Lecanicillium sp.GZWMJZ-847 from Gusuibu(Drynariae Rhizoma).Methods Lecanicillium sp.GZWMJZ-847 was cultured on the rice medium and its secondary metabolites were isolated and purified by Sephadex LH-20,silica gel column chromatography.Their structures were identified by the massspectroscopic analysis and NMR calculation.The activities of isolated compounds against pathogenic bacteria and yeast were assayed by nutrient broth dilution method,and that against pathogenic moulds were tested by plate method.Results Three new 3-decalinoyltetramic acids,lecanitetramic acids A-C(1-3),along with three known analogs including Sch 210971(4),Sch 210972(5)and Sch 213766(6)were obtained.Compounds1and 4-6showed antimicrobial activity against four human Gram-positive and one plant Gram-negative bacteria with the MIC values ranging from 7.81 to 125.00mmol/L.Compound 6showed anti-phytopathogenic fungi activities against Sclerotiniasclerotiorum and Fusarium graminearumwith IC50values of(22.05±0.62)and(55.54±1.72)μg/mL.Conclusion Lecanitetramic acids A-C(1-3)are new 3-decalinoyltetramic acids.Lecanicilliumsp.GZWMJZ-847 from Drynariae Rhizoma can produce antimicrobial 3-decalinoyltetramic acids against human-and plant-derived pathogenic microbes.

关 键 词：骨碎补 蜡蚧菌 次级代谢产物 吡咯烷-2 4-二酮 抑菌活性 蜡蚧特特拉姆酸A 蜡蚧特特拉姆酸B 蜡蚧特特拉姆酸C 

分 类 号：R284.1[医药卫生—中药学]