不同产区艾叶ISSR遗传多样性分析  

ISSR analysis on genetic diversity of Artemisiae Argyi Folium from different habitats

作　　者：余孟娟 赵博宇 董诚明[1] 李询 邢冰 李曼 YU Meng-juan;ZHAO Bo-yu;DONG Cheng-ming;LI Xun;XING Bing;LI Man(College of Pharmacy,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,郑州河南450046

出　　处：《中草药》2023年第13期4306-4311,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(81603232)。

摘　　要：目的对不同产区艾Artemisia argyi叶进行遗传多样性分析,为艾叶规范化种植及新品种选育工作奠定基础。方法采用ISSR分子标记技术对36个不同产区的艾叶样品进行扩增,然后利用POPGENEv1.32软件进行遗传学分析,得出它们的遗传特征系数,用Ntsyspc2.1对艾叶ISSR分子标记结果进行UPGMA聚类分析。结果筛选出16条引物,共扩增出条带402条,其中多态性条带398条,多态性百分比为99%;在对栽培种和野生种之间遗传距离分析后发现,两者之间的遗传一致度为0.9891,遗传距离为0.0110;对按纬度划分的4个区域艾资源进行遗传结构与遗传变异分析,4个区域间基因多样性指数(diversity index,Ht)为0.1927、遗传多样性指数(genetic diversity index,Hs)为0.1642、遗传变异指数(genetic variation index,Gst)为0.1479、基因流(gene flow,Nm)为2.8818,NTSYSpc软件对4个区域进行系统聚类分析,可将4个区位划分为2大类,且4区位相邻2个之间均具有一定的遗传相似性。结论艾叶具有极其丰富的遗传多样性,栽培种和野生种之间亲缘关系接近、遗传背景差异较小。艾叶其遗传特性随着纬度的变化呈现出一种递进式遗传扩散,其中河南南部居群多样性最为丰富,其次为河南中西部和河南中部,北部居群遗传多样性最低。Objective The genetic diversity analysis of Artemisia argyi in different producing areas will lay the foundation for the standardized planting and selection of new varieties of Artemisia argyi.Methods ISSR molecular marker technology was used to amplify artemisia sylvestris samples from 36 different production areas,and then genetic analysis was performed using POPGENE v1.32 software to obtain their genetic characteristic coefficients.Ntsyspc2.1 was used to perform ISSR molecular marker results UPGMA cluster analysis.Results 16 primers were selected,and a total of 402 bands were amplified,including 398 polymorphic bands,and the percentage of polymorphism was 99%..After analyzing the genetic distance between the wild species and the wild species,it was found that the genetic identity between the two is 0.9891 and the genetic distance is 0.0110.The diversity index(Ht)is 0.1927,the genetic diversity index(Hs)is 0.1642,the genetic variation index(Gst)is 0.1479,and the gene flow(Nm)is 2.8818.NTSYSpc software performs a systematic cluster analysis on 4 regions.The 4 loci are divided into 2 categories,and there are certain genetic similarities between the two adjacent 4 loci.Conclusion Artemisia oleifera is extremely rich in genetic diversity,the genetic relationship between cultivated and wild species is close,and the genetic background is small.The genetic characteristics of Artemisia argyi exhibit a progressive genetic spread with changes in latitude.Among them,southern Henan has the most abundant population diversity,followed by central and western Henan and central Henan,and northern population has the lowest genetic diversity.

关 键 词：艾叶 ISSR分子标记 遗传多样性 栽培 野生 不同纬度 

分 类 号：R286.12[医药卫生—中药学]