生、醋黄丝郁金治疗“肝郁气滞”型痛经大鼠的药效比较研究  

Effect of processing with vinegar on efficacy of Curcuma longa in treatment of dysmenorrhea in rats with syndrome of liver depression and Qi stagnation

作　　者：吴杰 曹马怡洁 彭颖[1] 东宝花 蒋云秀 朱鹏锦 胡昌江[1,2] 许润春 陈志敏[1] WU Jie;CAO Ma-yi-jie;PENG Ying;DONG Bao-hua;JIANG Yun-xiu;ZHU Peng-jin;HU Chang-jiang;XU Run-chun;CHEN Zhi-min(Sichuan Intangible Cultural Heritage Protection and Inheritance Base,State Key Laboratory of Southwest Characteristic Traditional Chinese Medicine Resources,College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;Key Laboratory of Quality and Efficacy Evaluation of Traditional Chinese Medicine Formula Granules of National Administration of Traditional Chinese Medicine,Sichuan New Green Pharmaceutical Technology Development Co.,Ltd.,Chengdu 610031,China)

机构地区：[1]成都中医药大学药学院西南特色中药资源国家重点实验室,四川省非物质文化遗产保护传承基地,四川成都610075 [2]四川新绿色药业科技发展有限公司,国家中医药管理局“中药配方颗粒质量与疗效评价重点研究室”,四川成都610031

出　　处：《中国中药杂志》2023年第13期3498-3507,共10页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(82204624);四川省重点研发项目(2023YFS0458);成都中医药大学西南特色中药资源重点实验室开放研究基金项目(2020QNJS005,2020JCRC014)。

摘　　要：该研究通过对比黄丝郁金醋炙前后对“肝郁气滞”型痛经模型大鼠药效及作用机制差异,探讨黄丝郁金“醋炙入肝”后增强“疏肝止痛”作用的炮制机制。参照痛经动物模型制备规范(草案)和慢性不可预见性应激法建立“肝郁气滞”型痛经大鼠病证结合模型。通过观测“肝郁气滞”型痛经大鼠体质量、脏器、脏脑指数、扭体潜伏期和扭体分数变化,检测各组大鼠血清肝功能六项、性激素、疼痛因子以及血液流变学等各项指标水平,比较生、醋黄丝郁金治疗“肝郁气滞”型痛经大鼠药效差异。在大鼠一般情况和行为学测试观察中,生品组和醋炙组体质量减轻缓慢,扭体潜伏期显著增加,扭体反应显著减少(P<0.05),其中生品组、醋炙组和阳性组大鼠扭体抑制率分别为33.780%、64.611%、62.466%,醋炙组扭体抑制率显著提高,表明醋黄丝郁金治疗作用更显著。在肝功能检测中,醋炙组ALT、ALP、AST、DBIL、TBIL水平显著降低(P<0.05)和ALB显著升高(P<0.05),表明醋炙增强了黄丝郁金对肝损伤的治疗作用。在性激素检测中,醋炙组血清E_(2)和OT水平显著降低(P<0.05),表明黄丝郁金醋炙后可调节大鼠机体性激素水平,减轻子宫平滑肌的活动和子宫的收缩,缓解痛经症状。在疼痛因子检测中,醋炙组的IL-6和AVP水平显著降低(P<0.05),β-EP水平显著升高(P<0.05),表明醋黄丝郁金通过调节机体疼痛因子水平,发挥镇痛效果。在血液流变学检测中,各给药组的全血黏度低切、中切、高切值,血浆黏度,全血还原黏度低切、高切值,红细胞积压和血沉方程K水平均显著降低(P<0.05),且黄丝郁金醋炙后改善血液程度显著优于黄丝郁金生品,表明黄丝郁金醋炙之后可增强改善机体组织局部血液流变学,提高临床疗效。黄丝郁金醋炙后可“入肝”,缓解心、肝、肾和子宫等脏器损伤程度,回调机体肝功能水平,并通过调节�This study compared the effects of Curcuma longa before and after processing with vinegar on the rat model of dysmenorrhea with the syndrome of liver depression and Qi stagnation to reveal the mechanism of vinegar processing in improving the role of C.longa in soothing liver and relieving pain.The rat model of dysmenorrhea with the syndrome of liver depression and Qi stagnation was established according to the Preparation of the Animal Model of Dysmenorrhea(Draft) and the chronic unpredictable stress me-thod.The changes in the body weight,organ indexes,writhing latency,writhing score,and serum levels of six liver function indicators,sex hormones,pain factors,and blood rheological indicators were measured to evaluate the efficacy of C.longa processed with vinegar or not in treating dysmenorrhea in the rats with syndrome of liver depression and qi stagnation.Compared with the model group,the C.longa group(processed with vinegar or not) showed slow weight loss,increase in writhing latency,and decrease in writhing response(P<0.05).The inhibition rates on writhing in raw C.longa,vinegar-processed C.longa,and positive groups were 33.780%,64.611%,and 62.466%,respectively.The significantly higher inhibition rate of the vinegar processing group indicated that vinegar-processed C.longa demonstrated more significant therapeutic effect.The vinegar-processed C.longa group showed lower levels of alanine aminotransferase(ALT),alkaline phosphatase(ALP),aspartate aminotransferase(AST),direct bilirubin(DBIL),and total bilirubin(TBIL) and higher level of albumin(ALB)(P<0.05),which indicated that vinegar processing enhanced the therapeutic effect of C.longa on liver injury.The serum levels of estradiol(E_2) and oxytocin(OT) were lower in the vinegar-processed C.longa group(P<0.05),indicating that the vinegar-processed C.longa could regulate the sex hormone levels,reduce the activity of uterine smooth muscle and contraction of uterus,and alleviate the symptoms of dysmenorrhea in rats.Moreover,the vinegar-processed C.longa group showe

关 键 词：黄丝郁金 醋炙入肝 “肝郁气滞”型痛经 药效 

分 类 号：R285.5[医药卫生—中药学]