矮紫堇中2个新的异喹啉生物碱  

Two new isoquinoline alkaloids from Corydalis hendersonii

作　　者：周晓春 马晓静 戈福星 刘长鑫 梁亚娜 高小力 柴兴云 ZHOU Xiao-chun;MA Xiao-jing;GE Fu-xing;LIU Chang-xin;LIANG Ya-na;GAO Xiao-li;CHAI Xing-yun(Modern Research Center for Traditional Chinese Medicine,School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学,北京中医药研究院中药现代研究中心,北京102488

出　　处：《中国中药杂志》2023年第13期3508-3515,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81774001);中央本级重大增减支项目(2060302)。

摘　　要：矮紫堇具有清热解毒、凉血、止泻、降血压功效,临床常用于治疗低氧引起血液中红细胞异常的高山多血症、脉管炎、消化系统的“木布”综合征和腹泻等。该研究通过正相硅胶柱、反相硅胶柱(ODS)、Sephadex LH-20及半制备HPLC等色谱方法从其乙醇提取物中分离得到9个化合物,其结构经波谱分析和与文献数据对比鉴定为hendersine H(1)、hendersine I(2)、去氢碎叶紫堇碱(dehydrocheilanthifoline,3)、普罗托品(protopine,4)、伊米任碱(izmirine,5)、6,7-methylenedioxy-1(2H)-isoquinolinone(6)、icariside D_(2)(7)、ethyl 4-(β-D-glucopyranosyloxy)-3-methoxybenzoate(8)、3-羟基-4-甲氧基苯甲酸(9),其中化合物1和2为新化合物,化合物7~9为首次从该属植物中报道。部分化合物经H9c2心肌细胞缺糖缺氧模型和条件上清诱导H9c2心肌细胞炎症模型进行活性筛选,结果显示,化合物1在20μmol·L^(-1)时对缺糖缺氧的H9c2心肌细胞具有一定的保护作用。化合物4和5在10μmol·L^(-1)时能抑制条件上清诱导的H9c2心肌细胞炎症。Corydalis hendersonii(CH) is a Tibetan folk medicine with the functions of clearing heat,detoxifying,cooling blood,checking diarrhea,and lowering blood pressure.It is often used to treat high altitude polycythemia,vasculitis,peptic ulcer,and diarrhea.Nine compounds were separated from the ethanol extract of CH by silica gel,ODS,Sephadex LH-20 chromatography and semi-preparative HPLC.Their structures were identified as hendersine H(1),hendersine I(2),dehydrocheilanthifoline(3),protopine(4),izmirine(5),6,7-methylenedioxy-1(2H)-isoquinolinone(6),icariside D_2(7),ethyl 4-(β-D-glucopyranosyloxy)-3-methoxybenzoate(8),3-hydroxy-4-methoxybenzoic acid(9),respectively,by the spectroscopic data analysis and comparison with those in the literature.Among them,compounds 1 and 2 are new isoquinoline alkaloids,and compounds 7-9 are reported the first time for Corydalis.The hypoglycemic model of H9c2 cardiomyocytes and the inflammatory model of H9c2 cardiomyocytes induced by conditional supernatant were employed to determine the activities of the above compounds.The results showed that 20 μmol·L^(-1) compound 1 had a protective effect on H9c2 cardiomyocytes and 10 μmol·L^(-1) compounds 4 and 5 inhibited H9c2 cardiomyocyte inflammation induced by conditional supernatant.

关 键 词：藏族药 矮紫堇 异喹啉生物碱 抗心肌缺血 抗炎 心肌细胞保护 

分 类 号：R285[医药卫生—中药学]