广东紫珠化学成分及止血活性研究  

Chemical constituents from Callicarpa kwangtungensis and their hemostatic activities

作　　者：薛淑田 刘伟 袁涛 XUE Shu-tian;LIU Wei;YUAN Tao(Key Laboratory of Natural Product Chemistry and Application at Universities of Education Department of Xinjiang Uygur Autonomous Region,School of Chemistry and Environmental Science,Yili Normal University,Yining 835000,China;College of Life Sciences,Jiangxi Normal University,Nanchang 330022,China)

机构地区：[1]伊犁师范大学化学与环境科学学院、新疆维吾尔自治区教育厅天然产物化学与应用重点实验室,新疆伊宁835000 [2]江西师范大学生命科学学院,江西南昌330022

出　　处：《中国中药杂志》2023年第14期3815-3825,共11页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(82060774);江西省“双千计划”项目(JXSQ2018101008);伊犁师范大学学实“高层次人才岗位”项目(YSXSGG22003)。

摘　　要：为了研究广东紫珠化学成分及其止血活性,采用MCI、反相ODS、Sephadex LH-20凝胶、半制备液相等色谱技术从广东紫珠活性部位中分离得到15个化合物,经多种波谱数据分析和文献比对,分别鉴定为3-O-α-L-rhamnopyranosyl-6-O-β-D-apiofuranosyl-4-O-E-caffeoyl-D-glucopyranoside(1)、3,6-O-α-L-dirhamnopyranosyl-4-O-E-caffeoyl-D-glucopyranoside(2)、β-OH-forsythoside B(3)、β-OH-poliumoside(4)、(+)-lyoniresinol-3α-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside(5)、(+)-lyoniresinol-3α-O-β-D-glucopyranoside(6)、(-)-lyoniresinol-3α-O-β-D-glucopyranoside(7)、kelampayoside A(8)、descaffeoylpoliumoside(9)、acteoside(10)、alyssonoside(11)、poliumoside(12)、isacteoside(13)、acetyl forsythoside B(14)、forsythoside B(15)。其中,化合物1和2为新化合物,化合物3和4为首次报道其核磁数据。以小鼠断尾模型,云南白药为阳性对照,生理盐水为阴性对照,对广东紫珠提取物以及量丰成分化合物12和15进行了止血活性测定。结果显示广东紫珠提取物以及2个主要成分均表现出显著的止血效果。This study aims to identify the chemical constituents from Callicarpa kwangtungensis and determine their activities.MCI,ODS,and Sephadex LH-20 chromatography and semi-preparative HPLC were employed to separate the chemical constituents.A total of 15 compounds were separated,and their structures were identified on the basis of spectroscopic analysis and comparison with the data in relevant literature.Specifically,the 15 compounds were 3-O-α-L-rhamnopyranosyl-6-O-β-D-apiofuranosyl-4-O-E-caffeoyl-D-glucopyranoside(1),3,6-O-α-L-dirhamnopyranosyl-4-O-E-caffeoyl-D-glucopyranoside(2),β-OH-forsythoside B(3),β-OH-poliumoside(4),(+)-lyoniresinol-3α-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside(5),(+)-lyoniresinol-3α-O-β-D-glucopyranoside(6),(-)-lyoniresinol-3α-O-β-D-glucopyranoside(7),kelampayoside A(8),descaffeoylpoliumoside(9),acteoside(10),alyssonoside(11),poliumoside(12),isacteoside(13),acetyl forsythoside B(14),and forsythoside B(15).Compounds 1 and 2 were novel,and the NMR data of compounds 3 and 4 were reported here for the first time.Furthermore,the hemostatic activities of the extract and abundant ingredients(compounds 12 and 15)of C.kwangtungensis were determined with Yunnan Baiyao as the positive control and normal saline as the negative control.The extract and compounds 12 and 15 significantly shortened the tail tip bleeding time in mice.

关 键 词：广东紫珠 化学成分 苯乙醇苷类 止血活性 

分 类 号：R284.1[医药卫生—中药学]