皮下植入聚乳酸/明胶/碳纤维多孔导电凝胶的生物相容性研究  

The biocompatibility of porous conductive gel-material of polylactic acid/gelatin/carbon fibers implanted subcutaneously

作　　者：校搏[1] 胡雪纯 刘健[2] 黄宇光[1] 许海燕[2] XIAO Bo;HU Xuechun;LIU Jian;HUANG Yuguang;XU Haiyan(Department of Anesthesiology,Peking Union Medical College Hospital,Chinese Academy of Medical Science&Peking Union Medical College,Beijing 100730;Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100005)

机构地区：[1]中国医学科学院北京协和医学院,北京协和医院麻醉科,北京100730 [2]中国医学科学院基础医学研究所,北京协和医学院基础学院,北京100005

出　　处：《北京生物医学工程》2023年第4期355-360,共6页Beijing Biomedical Engineering

基　　金：国家自然科学基金(81971366);中国医学科学院医学与健康科技创新工程项目(CIFMS 2021-I2M-1-006)资助。

摘　　要：目的采用小鼠皮下植入方法评价聚乳酸/明胶/碳纤维多孔导电凝胶(PG@CF)的生物相容性。方法将灭菌后的材料植入健康小鼠皮下,应用免疫组化与免疫荧光染色方法对支架周围组织的形态、炎症细胞浸润度、胶原纤维沉积厚度和平均新生血管面积进行观察与统计。结果多孔导电凝胶植入体内后可保持多孔结构;植入后28 d内,凝胶周围的炎症细胞浸润厚度和胶原沉积厚度均低于对照材料,其包囊中的平均血管面积显著高于对照材料。结论与对照材料相比,多孔导电凝胶引起的异物反应程度较低。本研究可为该导电凝胶在组织修复中的安全应用提供参考。Objective To evaluate the biocompatibility of the porous conductive gels composed of polylactic acid,gelatin,and carbon fibers(PG@CF)by subcutaneous implantation in mice.Methods After implanted subcutaneously in healthy mice,the morphology of the gels,the infiltration of inflammatory cells,the thickness of collagen for the encapsulation,and the average area of neovascular in the tissue around the gels were observed by using immunohistochemical and immunofluorescence staining.Results The porous structure of PG@CF could maintain in vivo within the testing period.During 28-day after the implantation,the inflammatory cell infiltration and the thickness of collagen deposition around the gels were less than those of the control.The average area of neovascular in the encapsulation was significantly larger than that of the control.Conclusions Compared with the control material,the foreign-body responses elicited by the porous conductive gels were lower,which indicated better safety of application in vivo.

关 键 词：组织工程 多孔导电支架 碳纤维 异物反应 

分 类 号：R318.08[医药卫生—生物医学工程]