基于脂质组学研究硒元素对妊娠期砷暴露大鼠脂代谢干预作用机制  

A Lipidomics-based Study on the Mechanism of Selenium Intervention on Lipid Metabolism in Arsenic-exposed Rats During Pregnancy

作　　者：邹晓杰 何俐 蔡丽静 王妍[1] 郑能兴 丁蕊[3,4] 吴衍 ZOU Xiaojie;HE Li;CAI Lijing;WANG Yan;ZHENG Nengxing;DING Rui;WU Yan(School of Public Health,Fujian Medical University,Fuzhou 350122,China;School of Public Health,Xiamen University,Xiamen 361102,China;College of Environmental and Resource Sciences/College of Carbon Neutral Modern Industry,Fujian Normal University,Fuzhou 350117,China;Fujian Key Laboratory of Pollution Control&Resource Reuse,Fuzhou 350117,China)

机构地区：[1]福建医科大学公共卫生学院,福建福州350122 [2]厦门大学公共卫生学院,福建厦门361102 [3]福建师范大学环境与资源学院/碳中和现代产业学院,福建福州350117 [4]福建省污染控制与资源循环利用重点实验室,福建福州350117

出　　处：《福建师范大学学报（自然科学版）》2023年第4期19-28,共10页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家自然科学基金青年基金资助项目(22006016);福建省自然科学基金资助项目(2022J01710)。

摘　　要：孕期雌鼠(GD0至GD20)经水暴露于质量浓度为0、0.1、1.0、10.0 mg·L^(-1)的As^(3+)中.其中高剂量组于GD15-GD19时进行硒干预.GD20时取血液和肝脏,并计算肝脏系数.电感耦合等离子体质谱检测血清和肝脏中的元素含量;液相色谱串联三重四极杆质谱进行拟靶向脂质组学检测;全自动生化分析仪检测血清生化指标.结果显示,高剂量砷暴露可诱发大鼠脂代谢紊乱,硒干预后会改变血清中脂质代谢过程.Pregnant female rats(GD0 to GD20) were exposed to As^(3+) in water at concentrations of 0,0.1,1.0,and 10.0 mg·L^(-1).The high-dose group underwent selenium intervention at GD15-GD19.Blood and liver samples were collected at GD20,and liver coefficients were calculated.Inductively coupled plasma mass spectrometry was used to detect the elemental contents in serum and liver;liquid chromatography tandem triple quadrupole mass spectrometry was used for pseudo-targeted lipidomics;and a fully automated biochemical analyzer was used to detect serum biochemical parameters.The results showed that high-dose As exposure induced disorders of lipid metabolism in rats,and Se intervention altered the process of lipid metabolism in serum.

关 键 词：砷 硒 脂代谢 脂质组学 

分 类 号：R115[医药卫生—公共卫生与预防医学]