黄芪联合川芎嗪减少游离真皮脂肪瓣体积损失的作用及机制研究  

Effect and mechanism of Astragalus membranaceus combined with Ligustrazine on reducing volume loss of free dermal fat flap

作　　者：杨欧欧[1] 罗华[1] 陈慧 杨慧芬[1] 詹晨妮 徐海滨[1] YANG Ou-ou;LUO Hua;CHEN Hui(Department of Breast Surgery,Hangzhou Hospital of Traditional Chinese Medicine,Hangzhou(310000),China)

机构地区：[1]杭州市中医院乳腺病科,杭州310000

出　　处：《中国中西医结合外科杂志》2023年第4期524-528,共5页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：浙江省中医药科技计划项目(2018ZA088)。

摘　　要：目的:研究中药黄芪联合川芎嗪在减少游离真皮脂肪瓣体积损失中的作用及其机理,探索最佳的辅助用药方案。方法:将80只雌性SD大鼠腹部的游离真皮脂肪瓣移植到胸部,成功建模。随后将大鼠分成4组(每组20只):对照组,每天腹腔注射生理盐水;黄芪注射液组,每天腹腔注射黄芪注射液(2 g/mL);川芎嗪注射液组,每天腹腔注射川芎嗪注射液(2.4 mg/mL);黄芪+川芎嗪组,每天腹腔注射含有黄芪注射液和川芎嗪注射液1:1的混合液,持续4周。每组在术后即刻、1周、2周和4周取出移植物,测定各时间点真皮脂肪瓣的重量和厚度、氧化损伤程度,并测定血管内皮细胞生长因子(VEGF)和高度糖基化的I型跨膜糖蛋白(CD34)的表达。结果:所有给药组都能增加游离真皮脂肪瓣的重量和厚度(P<0.001),提高超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活性,并降低真皮脂肪中丙二醛(MDA)的含量(P<0.05);所有给药组在第4周都显著增加了游离真皮脂肪瓣组织VEGF的表达,其中黄芪注射液联合川芎嗪注射液显著增加了CD34的表达(P<0.05)。结论:黄芪注射液和川芎嗪注射液有助于促使新生血管的形成,减少皮瓣缺血再灌注损伤,有助于延缓真皮游离脂肪萎缩。在体积维持上,黄芪注射液和川芎嗪注射液联合应用效果最佳。Objective To investigate the effect and mechanism of astragalus membranaceus combined with ligustrazine on reducing the volume loss of free dermal fat flap,and to explore the best adjuvant medication.Methods The free dermal fat flap from the abdomen of female SD rats was transplanted into the chest and successfully modeled.Then,the rats were divided into four groups(20 in each group):control group,daily intraperitoneal injection of normal saline;Astragalus membranaceus injection(AM)group was intraperitoneally injected with Astragalus injection(2 g/mL)every day;Ligustrazine injection group,daily intraperitoneal injection of ligustrazine injection(2.4 mg/mL);AM+ligustrazine group was intraperitoneally injected with a mixture of AM injection and ligustrazine injection 1:1 every day for four weeks.The grafts were removed immediately,1 week,2 weeks and 4 weeks after surgery.The weight and thickness of the dermal fat flap and the degree of oxidative damage were measured at each time point.The expressions of vascular endothelial cell growth factor(VEGF)and highly glycosylated type I transmembrane glycoprotein(CD34)were measured by immunohistochemistry and western blotting.Results All dosage groups can increase the weight of the free leather fat flap and thickness(P<0.001).The activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)was increased,and the content of malondialdehyde(MDA)in dermal fat was decreased(P<0.05).All dosage groups were increased significantly in 4 weeks for the free leather fat flap of tissue VEGF expression(P<0.05),including AM injection combined with ligustrazine injection significantly increased the expression of CD34(P=0.002).Conclusion Our research provides a novel insight into the effects of AM and ligustrazine injection on delaying the atrophy of free dermal fat.

关 键 词：黄芪 川芎嗪 游离真皮脂肪移植 血管内皮细胞生长因子 CD34 

分 类 号：R268[医药卫生—中医外科学]