针刺长强穴调控炎症反应改善脑性瘫痪大鼠运动和学习记忆能力的机制研究  

Mechanism of Acupuncture at Changqiang Acupoint(GV1)to Regulate Inflammatory Response and Improve Motor and Learning Memory Ability in Cerebral Palsy Rats

作　　者：张学君[1] 杨晓丹 赵锦燕 林晨捷 郑紫宁 洪慕尧 吴强[1] ZHANG Xuejun;YANG Xiaodan;ZHAO Jinyan;LIN Chenjie;ZHENG Zining;HONG Muyao;WU Qiang(College of Acupuncture and Moxibustion,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China;Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China;Fujian Provincial Key Laboratory of Integrative Medicine on Geriatrics,Fuzhou,Fujian 350122,China)

机构地区：[1]福建中医药大学针灸学院,福建福州350122 [2]福建中医药大学中西医结合研究院,福建福州350122 [3]福建省中西医结合老年性疾病重点实验室,福建福州350122

出　　处：《福建中医药》2023年第7期16-20,共5页Fujian Journal of Traditional Chinese Medicine

基　　金：福建省自然科学基金项目(2020J01743)。

摘　　要：目的从针刺长强穴对脑性瘫痪(简称脑瘫)大鼠大脑皮层和海马区NF-κB、NLRP3和Caspase-1蛋白表达及血清炎症因子水平的影响角度探讨其改善脑瘫大鼠运动和学习记忆功能的作用机制。方法采用改良Rice-Vannucci法制备大鼠脑瘫模型,造模成功后将18只7日龄新生SD大鼠按随机数字表法分为模型组、非穴组和长强组各6只,另挑选12只7日龄新生SD大鼠分为空白组和假手术组各6只。空白组不予任何处理,假手术组仅单纯分离其左侧颈总动脉,不进行结扎和剪断血管。造模后第1天开始,长强组针刺长强穴,非穴组取针刺“非穴”固定点(髂嵴上约15 mm和后正中线旁开约20 mm的交叉点),均不留针,连续干预5 d为1个疗程,疗程间休息2 d,共治疗2个疗程;空白组、假手术组和模型组均不予干预。干预后比较5组悬吊时间、斜坡时间、Morris水迷宫逃避潜伏期与穿越平台次数;采用ELISA法检测5组大鼠血清白细胞介素-6(IL-6)、白细胞介素-10(IL-10)和肿瘤坏死因子-α(TNF-α)含量;免疫组织化学法检测5组大鼠大脑皮层和海马CA1区核因子-κB(NF-κB)、NOD样受体热蛋白结构域3(NLRP3)和半胱氨酸蛋白酶-1(Caspase-1)蛋白表达水平。结果干预后与空白组比较,假手术组悬吊时间、斜坡时间、Morris水迷宫逃避潜伏期与穿越平台次数,血清IL-6、IL-10和TNF-α含量,大脑皮层和海马CA1区NF-κB、NLRP3和Caspase-1蛋白表达水平差异均无统计学意义(P均>0.05);干预后与空白组比较,模型组与非穴组斜坡时间、Morris水迷宫逃避潜伏期时间均明显增加,悬吊时间、穿越平台次数均明显减少,血清IL-6和TNF-α含量均升高,IL-10含量均降低,大脑皮层和海马CA1区NF-κB、NLRP3和Cas⁃pase-1蛋白表达水平均明显上升(P<0.05或0.01);干预后与模型组和非穴组比较,长强组斜坡时间、Morris水迷宫逃避潜伏期时间均明显减少,悬吊时间、穿越平台次数明显增加,血Objective:The effect of acupuncture at Changqiang acupoint(GV1)on the expression of NF-κB,NLRP3 and Caspase-1 protein and the level of serum inflammatory factors in cerebral cortex and hippocampus of cerebral palsy rats was investigated to explore its mechanism of improving the motor,learning and memory function of cerebral palsy rats.Methods:A rat cerebral palsy model was prepared using the modified Rice-Vannucci method.After successful modeling,eighteen 7-day old newborn SD rats were randomly divided into model group,non acupoint group,and Changqiang group,with 6 in each group.Additionally,twelve 7-day old newborn SD rats were selected and divided into blank group and sham operation group,with 6 in each group.The blank group received no treatment,while the sham operation group were only separated its left common carotid artery without ligation or cutting of blood vessels.Starting from the first day after modeling,the Changqiang group received acupuncture at Changqiang,while the non acupoint group received acupuncture at the"non acupoint"fixed point(about 15 mm above the iliac crest and about 20 mm lateral to the posterior midline),and had no needle retention time.Continuous intervention for 5 days was considered as one course of treatment,with 2 day break between courses.A total of 2 courses of treatment were conducted;the blank group,the sham surgery group,and the model group were not intervened.After intervention,the suspension time,slope time,Morris water maze escape latency,and number of crossing platforms among 5 groups were compared;detection of IL-6,IL-10 and TNF-αin serum content of the five groups of rats using ELISA method;immunohistochemical detection of NF-κB,NLRP3 and Caspase-1 protein expression levels in cerebral cortex and hippocampal CA1 region of rats in the five groups were conducted.Results:After intervention,compared with the blank group,the sham operation group's suspension time,slope time,Morris water maze escape latency and crossing platform frequency,and serum levels of IL-6,IL-10 an

关 键 词：脑性瘫痪 针刺 长强穴 运动功能 学习功能 炎症因子 

分 类 号：R245[医药卫生—针灸推拿学]