人参皂苷Rh2与吉非替尼联用对人肺腺癌NCI-H1975细胞的作用研究  

Study on the effffects of ginsenoside Rh2 combined with gefitinib on human lung adenocarcinoma NCI-H1975 cells

作　　者：丛中一[1] 吕泽旗 刘少薇 王镭 李升起 张贺 苏琪越 石少敏[3] 赵建军[3] CONG Zhongyi;LV Zeqi;LIU Shaowei;WANG Lei;LI Shengqi;ZHANG He;SU Qiyue;SHI Shaomin;ZHAO Jianjun(College of Pharmacy,Jilin University,Changchun 130021,China;Institute of Respiratory Health,the First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510120,China;Department of Respiratory Medicine,China-Japan Union Hospital of Jilin University,Changchun 130033,China)

机构地区：[1]吉林大学药学院,长春130021 [2]广州医科大学附属第一医院呼吸健康研究院,广州510120 [3]吉林大学中日联谊医院呼吸内科,长春130033

出　　处：《长春中医药大学学报》2023年第8期865-869,共5页Journal of Changchun University of Chinese Medicine

基　　金：吉林省科技发展计划项目(20200201393JC)。

摘　　要：目的探讨人参皂苷Rh2与吉非替尼联用对人肺腺癌细胞的体外作用,以期为非小细胞肺癌治疗研究提供理论依据。方法体外培养人肺腺癌NCI-H1975细胞,CCK-8法检测不同浓度人参皂苷和吉非替尼作用后的细胞活力,计算半抑制浓度(IC_(50)),然后用同样的方法检测人参皂苷Rh2与吉非替尼联用对细胞活力的影响;分别用PI染色法、Annexin V-FITC/PI双染法和JC-1法检测人参皂苷Rh2、吉非替尼单药和联合用药对细胞周期、凋亡和线粒体膜电位的影响。结果人参皂苷Rh2和吉非替尼单药对NCI-H1975细胞的作用呈剂量依赖性和时间依赖性,作用24、48、72 h的IC_(50),Rh2分别为57.90、41.38、32.62μg·mL^(-1),吉非替尼分别为56.50、22.80、13.19μmol·L^(-1);两药以IC_(50)剂量联用作用72 h后,与各单药组比较,联合用药组细胞活力显著降低(P<0.01),细胞周期无明显阻滞,细胞凋亡率显著升高(P<0.01),细胞线粒体膜电位显著降低(P<0.01)。结论人参皂苷Rh2和吉非替尼联用可显著抑制NCI-H1975细胞活力和诱导细胞凋亡,其作用优于单独用药。Objective To explore the effect of ginsenoside Rh2 combined with gefitinib on human lung adenocarcinoma cells in vitro,so as to provide theoretical basis for research on the treatment of non-small cell lung cancer.Methods Human lung adenocarcinoma NCI-H1975 cells were cultured in vitro,and the viability of cells was detected by CCK-8 assay after the treatment of ginsenosides and gefitinib at different concentrations.The half maximal inhibitory concentration(IC_(50))was calculated.Then the same method was used to detect the effect of ginsenoside Rh2 combined with gefitinib on cell viability.The effects of ginsenoside Rh2,gefitinib alone and their combination on cell cycle,apoptosis,and mitochondrial membrane potential were detected using PI staining,annexin V-FITC/PI double staining,and JC-1 method,respectively.Results The effects of ginsenoside Rh2 and gefitinib alone on NCI-H1975 cells were dose-dependent and time-dependent.After 24 hours,48 hours and 72hours treatment with Rh2 and gefitinib,the IC_(50) of Rh2 was 57.90μg·mL^(-1),41.38μg·mL^(-1)and 32.62μg·mL^(-1)respectively,while the IC_(50) of gefitinib was 56.50μg·mL^(-1),22.80μg·mL^(-1)and 13.19μg·mL^(-1)respectively.After 72 hours of treatment with the combination of two drugs of IC_(50) dosage,compared with the single drug users,the cell viability of combined drug users was significantly decreased(P<0.01),the cell cycle was not significantly blocked,the cell apoptosis rate was significantly increased(P<0.01),and the cell mitochondrial membrane potential was significantly decreased(P<0.01).Conclusion The combination of ginsenoside Rh2 and gefitinib can significantly inhibit the viability of NCI-H1975 cells and induce apoptosis of these cells,which is superior to that of single drug treatment.

关 键 词：人参皂苷RH2 吉非替尼 肺癌 凋亡 

分 类 号：R965[医药卫生—药理学]