基于网络药理学探讨益肾蠲痹丸治疗类风湿关节炎的作用机制  

Network pharmacology based research into mechanism of action of Yishen Juanbi Wan in treating rheumatoid arthritis

作　　者：时一鸣 姜平 蒋恬 朱婉华 何东仪[1,2] SHI Yiming;JIANG Ping;JIANG Tian;ZHU Wanhua;HE Dongyi(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Guanghua Hospital Affiliated toShanghai University of Traditional Chinese Medicine,Shanghai 200052,China;Nantong Liangchun Hospital ofTraditional Chinese Medicine,Nantong,Jiangsu 226009,China)

机构地区：[1]上海中医药大学,上海201203 [2]上海中医药大学附属光华医院,上海200052 [3]南通良春中医医院,江苏南通226009

出　　处：《上海中医药杂志》2023年第8期68-73,共6页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家中医药管理局区域中医(专科)诊疗中心建设项目(2018-2022);国家中医药管理局第七批全国名老中医药专家学术经验继承项目(国中医药人教发〔2022〕76号);上海市卫健委华东片区中西医结合关节病专科联盟项目(ZY〔2021-2023〕-0302);上海市何东仪名中医工作室建设项目(SHGZS-202220)。

摘　　要：目的采用网络药理学方法探讨益肾蠲痹丸治疗类风湿关节炎(RA)的分子作用机制。方法采用中药系统药理学数据库与分析平台(TCMSP)、中医药整合药理学研究平台(TCMIP)、本草组鉴(HERB)和中医药百科全书数据库(ETCM)获取益肾蠲痹丸的有效成分,并借助SwissTargetPrediction数据库对有效成分的作用靶点进行预测;在人类基因综合数据库(GeneCards)、人类疾病相关基因与突变位点信息数据库(DisGeNET)、在线人类孟德尔遗传数据库(OMIM)、药物靶标数据库(TTD)中搜索RA相关基因。将益肾蠲痹丸的作用靶点和与RA相关靶点进行映射交集,获得二者的交集靶点,借助STRING数据库构建蛋白互作(PPI)网络;采用Cytoscape 3.7.2软件筛选出核心靶点,采用Cytoscape 3.9软件进行基因本体(GO)、京都基因与基因组百科全书(KEGG)信号通路分析。结果共获得益肾蠲痹丸治疗RA的有效成分185个,其中关键成分为山柰酚、木犀草素、槲皮素和黄藤素,核心作用靶点为肿瘤坏死因子(TNF)、白介素-6(IL-6)、白介素-1β(IL-1β)、血管内皮生长因子A(VEGFA)、信号转导及转录激活蛋白3(STAT3)基因等。KEGG信号通路主要涉及TNF、丝裂原活化蛋白激酶(MAPK)、白介素-17(IL-17)、VEGF、核因子-κB(NF-κB)信号通路及RA疾病信号通路等。结论益肾蠲痹丸治疗RA具有多成分、多靶点、多通路协同作用优势,可通过调控相关信号通路调节机体免疫功能、影响细胞凋亡、抑制炎症反应。Objective To investigate the molecular mechanism of Yishen Juanbi Wan in the treatment of rheumatoid arthritis(RA)based on a network pharmacology approach.Methods Active components of Yishen Juanbi Wan were obtained from the TCMSP、TCMIP、HERB and ETCM databases,and their targets of active components were predicted using the SwissTarget Prediction database.RA-related genes were retrieved from databases including GeneCards,DisGeNET、OMIM and TTD databases.The targets of Yishen Juanbi Wan and the targets related to RA were mapped to obtain the intersection targets,PPI networks were constructed with the help of STRING database,the key targets of Yishen Juanbi Wan in treating RA were screened using Cytoscape 3.7.2 software,and GO and KEGG enrichment analyses were performed with Cytoscape 3.9 software.Results A total of 185 active components were obtained for the treatment of RA,including those key components like kaempferol,luteolin,quercetin and palmatine,and the core gene targets were TNF,IL⁃6,IL⁃1β,VEGFA,and STAT3,etc.KEGG analysis showed that the main signaling pathways involved were TNF,MAPK,IL-17,VEGF,NF-κB,and rheumatoid arthritis.Conclusion With the advantages of multi-component,multi-target and multi�pathway synergistic effects in the treatment of RA,Yishen Juanbi Wan can effectively regulate the body's immune function,affect apoptosis and inhibit inflammatory response by regulating relevant signaling pathways.

关 键 词：类风湿关节炎 益肾蠲痹丸 中医药疗法 网络药理学 作用机制 

分 类 号：R285[医药卫生—中药学]