Mitigating microbiological risks of potential pathogens carrying antibiotic resistance genes and virulence factors in receiving rivers:benefits of wastewater treatment plant upgrade  

作　　者：Guannan Mao Donglin Wang Yaohui Bai Jiuhui Qu 

机构地区：[1]Key Laboratory of Drinking Water Science and Technology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China [2]State Key Laboratory of Tibetan Plateau Earth System,Environment and Resources(TPESER),Institute of Tibetan Plateau Research,Chinese Academy of Sciences,Beijing 100101,China [3]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Frontiers of Environmental Science & Engineering》2023年第7期47-55,共9页环境科学与工程前沿（英文）

基　　金：the National Natural Science Foundation of China(42101128,51578537,and 51778603);Chinese Academy of Sciences(QYZDY-SSW-DQC004).

摘　　要：Wastewater treatment plants(WWTPs)with additional tertiary ultrafiltration membranes and ozonation treatment can improve water quality in receiving rivers.However,the impacts of WWTP upgrade(WWTP-UP)on pathogens carrying antibiotic resistance genes(ARGs)and virulence factors(VFs)in rivers remain poorly understood.In this study,ARGs,VFs,and their pathogenic hosts were investigated in three rivers impacted by large-scale WWTP-UP.A five-year sampling campaign covered the periods before and after WWTP-UP.Results showed that the abundance of total metagenome-assembled genomes(MAGs)containing both ARGs and VFs in receiving rivers did not decrease substantially after WWTP-UP,but the abundance of MAGs belonging to pathogenic genera that contain both ARGs and VFs(abbreviated as PAVs)declined markedly.'Genome-resolved metagenomics further revealed that WWTP-UP not only reduced most types of VFs and ARGs in PAVs,but also effectively eliminated efflux pump and nutritional VFs carried by PAVs in receiving rivers.WWTP-UP narrowed the pathogenic host ranges of ARGs and VFs and mitigated the cooccurrence of ARGs and VFs in receiving rivers.These findings underline the importance of WWTPUP for the alleviation of pathogens containing both ARGs and VFs in receiving rivers。

关 键 词：Wastewater treatment plant upgrade Antibiotic resistance genes(ARGs) Virulencefactors(VFs) Geneco-occurrence Genome-centric analysis 

分 类 号：X703[环境科学与工程—环境工程]