氢吗啡酮对大鼠的脑保护作用  

Cerebral protective effect of hydromorphone in rats

作　　者：李玥阳 方刚[1] 高满海[2] 李荣[2] 王静[2] LI Yueyang;FANG Gang;GAO Manhai;LI Rong;WANG Jing(Baotou Medical College,Baotou 014040,China;Department of Anesthesiology,the First Affiliated Hospital of Baotou Medical College,Inner Mongolia University of Science and Technology Baotou Medical College,Baotou 014010,China)

机构地区：[1]包头医学院,包头014040 [2]内蒙古科技大学包头医学院第一附属医院麻醉科,包头014010

出　　处：《广州医药》2023年第7期10-15,共6页Guangzhou Medical Journal

基　　金：包头市卫生健康委科技计划项目(wsjkwkj001)。

摘　　要：目的探讨氢吗啡酮对大鼠脑缺血再灌注损伤的影响。方法45只SD雄性大鼠随机分成3组:假手术组(Sham组)、脑缺血再灌注组(I/R组)和氢吗啡酮组(HM组)。采用Zea-Longa改良线拴法构建动物模型,再灌注24 h后,Zea-Longa评分法评价神经功能;TTC染色检测脑梗死体积;苏木精-伊红(HE)和Nissl染色观察海马神经元病理变化,Tunel染色观察细胞凋亡情况,Western blot、qPCR检测凋亡相关因子B淋巴细胞瘤(Bcl)-2、Bcl-2相关X蛋白(Bax)和半胱氨酸蛋白酶(Caspase)-3蛋白和mRNA表达量。结果与I/R组相比,HM组神经功能评分下降和脑梗死面积减小(P<0.05),Tunel阳性细胞数量减少(P<0.05),Bax和Caspase-3蛋白mRNA表达量减少,而Bcl-2表达量显著增加(P<0.05)。结论氢吗啡酮具有神经保护作用,可减轻大鼠脑缺血再灌注损伤。Objective To investigate the effect of hydromorphone on cerebral ischemia-reperfusion injury in rats.Methods Forty-five SD male rats were randomly divided into three groups:sham-operated group(Sham group),cerebral ischemia-reperfusion group(I/R group)and hydromorphone group(HM group).The animal models were constructed using the Zea-Longa modified line tethering method,and neurological function was evaluated by the Zea-Longa score after 24 h of reperfusion.TTC staining was used to detect the volume of cerebral infarction,hematoxylin-eosin(HE)and Nissl staining were used to observe the pathological changes of hippocampal neurons,and Tunel staining was used to observe apoptosis,Western blot,qPCR were used to detect apoptosis Bcl-2,Bcl-2-associated X protein(Bax)and cysteine protease(Caspase)-3 protein and mRNA expression.Results Compared with the I/R group,the HM group showed lower neurological function scores and cerebral infarct area(P<0.05),smaller number of Tunel-positive cells(P<0.05),less mRNA expression of Bax and Caspase-3 proteins and significantly higher expression of Bcl-2(P<0.05).Conclusions Hydromorphone has neuroprotective effects and can reduce cerebral ischemia-reperfusion injury in rats.

关 键 词：氢吗啡酮 脑缺血-再灌注 大鼠 海马神经元 

分 类 号：R965[医药卫生—药理学]